IF (Immunofluorescence)
(Immunofluorescence staining of MDA-MB-231 cells with anti-PD-L1 (AAA72282) 332M7 Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells stained with the chimeric rabbit IgG version of 332M7 (AAA72282-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72282-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Human PD-L1 Antibody | anti-PD-L1 antibody
Anti-PD-L1 [332M7], Human IgG1
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse, Cynomolgus Monkey
Applications
Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
PD-L1; N/A; Anti-PD-L1 [332M7], Human IgG1; Anti-PD-L1 [332M7]; CD274; PDL1; B7-H1; B7H1; B7 homolog 1; hPD-L1; PDCD1L1; PDCD1LG1; PDCD1 ligand 1; Programmed cell death 1 ligand 1; Programmed death ligand 1; anti-PD-L1 antibody
Host
Human
Reactivity
Human, Mouse, Cynomolgus Monkey
Isotype
Human IgG1, Kappa
Clone Number
332M7
Specificity
This antibody strongly binds human and cynomolgus monkey PD-L1 and shows weak binding for mouse PD-L1. PD-L1 is highly expressed in many human tumors.. PD-L1 exerts an immune suppressive signal through binding to PD-1 and B7.1, and tumor expression of PD-L1 can mediate cancer immune evasion. Blocking this pathway, therefore provides a rationale for cancer immunotherapy.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PD-L1 antibody
Blocking, FCM/FACS (Flow Cytometry)
Immunogen
The original antibody was generated by immunizing BALB/c mice with recombinant human PD-L1.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluorescence staining of MDA-MB-231 cells with anti-PD-L1 (AAA72282) 332M7 Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells stained with the chimeric rabbit IgG version of 332M7 (AAA72282-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72282-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of MDA-MB-231 cells with anti-PD-L1 (AAA72282) 332M7 Immunofluorescence analysis of paraformaldehyde fixed MDA-MB-231 cells stained with the chimeric rabbit IgG version of 332M7 (AAA72282-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72282-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-PD-L1 antibody 332M7 (AAA72282). Paraformaldehyde fixed MDA-MB-231 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 332M7 (AAA72282-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using the Anti-PD-L1 antibody 332M7 (AAA72282). Paraformaldehyde fixed MDA-MB-231 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody or the rabbit IgG version of 332M7 (AAA72282-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-PD-L1 antibody
The binding characterization of this antibody towards human, mouse and cynomolgus monkey PD-L1 was done using flow cytometry. This antibody was shown to block the binding of hPD-1-Fc protein to hPD-L1 expressed on the surface of the HEK-293T cells at a concentration of 10 ug/ml. The mouse version of this antibody was reported to inhibit patient derived melanoma tumor in tumor-bearing mice. There was also found an increase in the gene expression of CD8 and IFN-gamma in tumor samples from mice treated with mouse version of this antibody (US20180346571).
References
US20180346571 PMID:
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,275 Da
NCBI Official Full Name
programmed cell death 1 ligand 1 isoform b
NCBI Official Synonym Full Names
CD274 molecule
NCBI Official Symbol
CD274
NCBI Official Synonym Symbols
B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
NCBI Protein Information
programmed cell death 1 ligand 1; B7 homolog 1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1
UniProt Protein Name
Programmed cell death 1 ligand 1
UniProt Gene Name
CD274
UniProt Synonym Gene Names
B7H1; PDCD1L1; PDCD1LG1; PDL1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7-H1
UniProt Entry Name
PD1L1_HUMAN
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Product Notes
The PD-L1 cd274 (Catalog #AAA72282) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-PD-L1 [332M7], Human IgG1 reacts with Human, Mouse, Cynomolgus Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's PD-L1 can be used in a range of immunoassay formats including, but not limited to, Blocking, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the PD-L1 cd274 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-L1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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