Standard Curve (Sample)
(Fig.1. Human Peptidylprolyl cis-trans isomerase A-like 4G (PPIAL4G) Standard Curve.)
Human Peptidylprolyl cis-Trans Isomerase A-Like 4G (PPIAL4G) ELISA Kit | PPIAL4G elisa kit
Human Peptidylprolyl cis-Trans Isomerase A-Like 4G (PPIAL4G) ELISA Kit
Gene Names
PPIAL4C; COAS-2
Reactivity
Human
Synonyms
Peptidylprolyl cis-Trans Isomerase A-Like 4G (PPIAL4G); N/A; Human Peptidylprolyl cis-Trans Isomerase A-Like 4G (PPIAL4G) ELISA Kit; PPIAL4G; RP6-206I17.5; BX284650.5; FLJ16804; MGC163248; MGC163250; PPIAL4G elisa kit
Reactivity
Human
Specificity
Human Peptidylprolyl cis-trans isomerase A-like 4G (PPIAL4G) ELISA Kit has high sensitivity and excellent specificity for detection of Human PPIAL4G. No significant cross-reactivity or interference between Human PPIAL4G and analogues was observed.
Sequence Length
164
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Peptidylprolyl cis-trans isomerase A-like 4G (PPIAL4G) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Peptidylprolyl cis-trans isomerase A-like 4G (PPIAL4G) Standard Curve.)
Related Product Information for PPIAL4G elisa kit
Description: This Human Peptidylprolyl cis-trans isomerase A-like 4G (PPIAL4G) ELISA Kit employs a two-site sandwich ELISA to quantitate PPIAL4G in samples. An antibody specific for PPIAL4G has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPIAL4G present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPIAL4G is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPIAL4G bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: PPIAL4G belongs to the cyclophilin-type PPIase family. PPIase A subfamily. Contains 1 PPIase cyclophilin-type domain, PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIases are ubiquitous in living organisms. While 3 families of PPIases, cyclophilin (CyP), FK506 binding protein (FKBP) and parvulin (Pvn), have been studied in detail in Eukarya and Bacteria (eubacteria), little is known about archaeal PPIases. Among 2 cyclophilins found in Archaea, only Halobacterium cyclophilin (HcCyP19) has been characterized. It is a cyclosporin A (CsA) sensitive CyP with a MW of 19.4kDa. The PPIase activity and CsA sensitivity of this CyP is higher at higher salt concentration in the medium. No parvulin or its homolog has been found in Archaea.
Background: PPIAL4G belongs to the cyclophilin-type PPIase family. PPIase A subfamily. Contains 1 PPIase cyclophilin-type domain, PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIases are ubiquitous in living organisms. While 3 families of PPIases, cyclophilin (CyP), FK506 binding protein (FKBP) and parvulin (Pvn), have been studied in detail in Eukarya and Bacteria (eubacteria), little is known about archaeal PPIases. Among 2 cyclophilins found in Archaea, only Halobacterium cyclophilin (HcCyP19) has been characterized. It is a cyclosporin A (CsA) sensitive CyP with a MW of 19.4kDa. The PPIase activity and CsA sensitivity of this CyP is higher at higher salt concentration in the medium. No parvulin or its homolog has been found in Archaea.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase A-like 4G
NCBI Official Synonym Full Names
peptidylprolyl isomerase A like 4C
NCBI Official Symbol
PPIAL4C
NCBI Official Synonym Symbols
COAS-2
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase A-like 4A/B/C
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase A-like 4G
UniProt Gene Name
PPIAL4G
UniProt Synonym Gene Names
PPIase A-like 4G
UniProt Entry Name
PAL4G_HUMAN
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Product Notes
The Human PPIAL4G ppial4g (Catalog #AAA331372) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331372 ELISA Kit recognizes Human PPIAL4G. It is sometimes possible for the material contained within the vial of "Peptidylprolyl cis-Trans Isomerase A-Like 4G (PPIAL4G), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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