Standard Curve (Sample)
(Fig.1. Human Perforin-1 (PRF1/PFP) Standard Curve.)
Human Perforin-1 (PRF1/PFP) ELISA Kit | PRF1/PFP elisa kit
Human Perforin-1 (PRF1/PFP) ELISA Kit
Gene Names
PRF1; P1; PFP; HPLH2
Reactivity
Human
Synonyms
Perforin-1 (PRF1/PFP); N/A; Human Perforin-1 (PRF1/PFP) ELISA Kit; PRF1; RP11-710A11.3; FLH2; HPLH2; MGC65093; P1; PFN1; PFP; OTTHUMP00000019759; cytolysin; lymphocyte pore forming protein; perforin 1; PRF1/PFP elisa kit
Reactivity
Human
Specificity
Human Perforin-1 (PRF1/PFP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRF1. No significant cross-reactivity or interference between Human PRF1 and analogues was observed.
Sequence Length
555
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Perforin-1 (PRF1/PFP) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Perforin-1 (PRF1/PFP) Standard Curve.)
Related Product Information for PRF1/PFP elisa kit
Description: This Human Perforin-1 (PRF1/PFP) ELISA Kit employs a two-site sandwich ELISA to quantitate PRF1 in samples. An antibody specific for PRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Perforin is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.
Background: Perforin is a cytolytic protein found in the granules of CD8 T-cells and NK cells. Perforin is a cytolytic protein found in the granules of Cytotoxic T lymphocytes (CTLs) and NK cells. Upon degranulation, perforin inserts itself into the target cell's plasma membrane, forming a pore. The lytic membrane-inserting part of perforin is the MACPF domain. This region shares homology with cholesterol-dependent cytolysins from Gram-positive bacteria. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
perforin-1
NCBI Official Synonym Full Names
perforin 1
NCBI Official Symbol
PRF1
NCBI Official Synonym Symbols
P1; PFP; HPLH2
NCBI Protein Information
perforin-1
UniProt Protein Name
Perforin-1
UniProt Gene Name
PRF1
UniProt Synonym Gene Names
PFP; P1; PFP
UniProt Entry Name
PERF_HUMAN
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Product Notes
The Human PRF1/PFP prf1 (Catalog #AAA331348) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331348 ELISA Kit recognizes Human PRF1/PFP. It is sometimes possible for the material contained within the vial of "Perforin-1 (PRF1/PFP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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