Standard Curve (Sample)
Human PLA2G4A ELISA Kit | PLA2G4A elisa kit
Human PLA2G4A PicoKine ELISA Kit
Gene Names
PLA2G4A; GURDP; cPLA2; PLA2G4; cPLA2-alpha
Reactivity
Human
Synonyms
PLA2G4A; N/A; Human PLA2G4A PicoKine ELISA Kit; Human PLA2G4A; phospholipase A2 group IVA; Cytosolic phospholipase A2; Cpla2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase; Lysophospholipase; CPLA2; PLA2G4; PLA2G4A elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human PLA2G4A
Sequence Length
749
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
<50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months. Store at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for PLA2G4A elisa kit
Principle of the Assay: The Picokine™ Human PLA2G4A Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human PLA2G4A with a 96-well strip plate that is pre-coated with antibody specific for PLA2G4A. The detection antibody is a biotinylated antibody specific for PLA2G4A. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human PLA2G4A with immunogen: Expression system for standard: NSO, Immunogen sequence: M1-A749. The kit is analytically validated with ready to use reagents. To measure Human PLA2G4A, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human PLA2G4A in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human PLA2G4A in the sample.!!Background/Introduction: Cytosolic phospholipase A2 is an enzyme that in humans is encoded by the PLA2G4A gene. It is mapped to 1q31.1. This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca2+ levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytosolic phospholipase A2 isoform 1
NCBI Official Synonym Full Names
phospholipase A2 group IVA
NCBI Official Symbol
PLA2G4A
NCBI Official Synonym Symbols
GURDP; cPLA2; PLA2G4; cPLA2-alpha
NCBI Protein Information
cytosolic phospholipase A2
UniProt Protein Name
Cytosolic phospholipase A2
UniProt Gene Name
PLA2G4A
UniProt Synonym Gene Names
CPLA2; PLA2G4; cPLA2
UniProt Entry Name
PA24A_HUMAN
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Product Notes
The Human PLA2G4A pla2g4a (Catalog #AAA125272) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125272 ELISA Kit recognizes Human PLA2G4A. It is sometimes possible for the material contained within the vial of "PLA2G4A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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