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IHC (Immunohistochemistry)
(AAA322333 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit c-PLA2 Polyclonal Antibody | anti-PLA2G4A antibody
c-PLA2 Antibody
Gene Names
PLA2G4A; cPLA2; PLA2G4; cPLA2-alpha
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
c-PLA2, Antibody; c-PLA2 Antibody; Calcium dependent phospholipid binding protein; CPLA 2; cPLA2 alpha; cPLA2; Cytosolic phospholipase A2; Cytosolic phospholipase A2 group IVA; Lysophospholipase; MGC126350; PA24A_HUMAN; Phosphatidylcholine 2 acylhydrolase; Phosphatidylcholine 2-acylhydrolase; Phospholipase A2 group 4 A; Phospholipase A2 group IVA (cytosolic calcium dependent); Phospholipase A2 group IVA; PhospholipaseA2; PLA2G4; pla2g4a; anti-PLA2G4A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
c-PLA2 antibody detects endogenous levels of total c-PLA2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
689
Applicable Applications for anti-PLA2G4A antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human c-PLA2
Subcellular Location
Cytoplasm. Cytoplasmic Vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
Tissue Specificity
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Bovine (82%), Horse (82%), Sheep (82%), Rabbit (91%), Dog (82%), Xenopus (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA322333 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA322333 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of c-PLA2 expression in TNF-a treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of c-PLA2 expression in TNF-a treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry)
(AAA322333 at 1/100 staining human colon tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at116 degree)
IHC (Immunohiostchemistry)
(AAA322333 at 1/100 staining human colon tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at116 degree)
IF (Immunofluorescence)
(AAA322333 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA322333 staining A549 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-PLA2G4A antibody
Description: cPLA2 a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid.
Function: Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
Subunit Structure: Interacts with KAT5.
Post-translational Modifications: Activated by phosphorylation at both Ser-505 and Ser-727.
Similarity: The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+.
Function: Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
Subunit Structure: Interacts with KAT5.
Post-translational Modifications: Activated by phosphorylation at both Ser-505 and Ser-727.
Similarity: The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 110 kDa
Predicted: 86 kDa
Predicted: 86 kDa
NCBI Official Full Name
cytosolic phospholipase A2 isoform 2
NCBI Official Synonym Full Names
phospholipase A2 group IVA
NCBI Official Symbol
PLA2G4A
NCBI Official Synonym Symbols
cPLA2; PLA2G4; cPLA2-alpha
NCBI Protein Information
cytosolic phospholipase A2
UniProt Protein Name
Cytosolic phospholipase A2
UniProt Gene Name
PLA2G4A
UniProt Synonym Gene Names
CPLA2; PLA2G4; cPLA2
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Product Notes
The PLA2G4A pla2g4a (Catalog #AAA322333) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The c-PLA2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-PLA2 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PLA2G4A pla2g4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-PLA2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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