WB (Western Blot)
(Anti-PLA2G4A antibody, AAA45409, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: A549 Cell LysateLane 4: COLO320 Cell Lysate)
Rabbit Phospholipase A2 Polyclonal Antibody | anti-PLA2G4A antibody
Anti-Phospholipase A2 antibody
Gene Names
PLA2G4A; PLA2G4; cPLA2-alpha
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Phospholipase A2, Antibody; Anti-Phospholipase A2 antibody; Cytosolic phospholipase A2; phospholipase A2, group IVA(cytosolic, calcium-dependent); Calcium dependent phospholipid binding protein antibody; CPLA 2 antibody; cPLA2 alpha antibody; cPLA2 antibody; Cytosolic phospholipase A2 antibody; Cytosolic phospholipase A2 group IVA antibody; Lysophospholipase antibody; MGC126350 antibody; PA24A_HUMAN antibody; Phosphatidylcholine 2 acylhydrolase antibody; Phosphatidylcholine 2-acylhydrolase antibody; Phospholipase A2 group 4 A antibody; Phospholipase A2 group IVA(cytosolic calcium dependent) antibody; Phospholipase A2 group IVA antibody; PhospholipaseA2 antibody; PLA2G4 antibody; pla2g4a antibody; anti-PLA2G4A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
749
Applicable Applications for anti-PLA2G4A antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Phospholipase A2 (686-700aa PNQAFKRLHDLMHFN), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-PLA2G4A antibody, AAA45409, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: A549 Cell LysateLane 4: COLO320 Cell Lysate)
WB (Western Blot)
(Anti-PLA2G4A antibody, AAA45409, Western blottingLane 1: Rat Testis Tissue LysateLane 2: Rat Brain Tissue LysateLane 3: A549 Cell LysateLane 4: COLO320 Cell Lysate)
Related Product Information for anti-PLA2G4A antibody
Description: Rabbit IgG polyclonal antibody for Cytosolic phospholipase A2(PLA2G4A) detection. Tested with WB in Human, Mouse, Rat.
Background: PLA2G4A(Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al.(1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al.(1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands(Skorecki, 1995).
Background: PLA2G4A(Phospholipase A2, Group IVA), is an enzyme that in humans is encoded by the PLA2G4A gene. Tay et al.(1995) mapped the PLA2G4A gene to rat chromosome 13 by PCR-based intercross genotyping and to human 1q25 by fluorescence in situ hybridization. By site-directed mutagenesis and biochemical analysis of the recombinant protein, Sharp et al.(1994) determined that ser228 participates in the catalytic mechanism of cPLA2 and that both the phospholipase A2 and the lysophospholipase activities are catalyzed by the same active site residue(s). PLA2G4A, the cytosolic phospholipase A2, appears to subserve transmembrane signaling responses to extracellular ligands(Skorecki, 1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
85,239 Da
NCBI Official Full Name
Cytosolic phospholipase A2
NCBI Official Synonym Full Names
phospholipase A2, group IVA (cytosolic, calcium-dependent)
NCBI Official Symbol
PLA2G4A
NCBI Official Synonym Symbols
PLA2G4; cPLA2-alpha
NCBI Protein Information
cytosolic phospholipase A2; cPLA2; lysophospholipase; phospholipase A2 group IVA; phosphatidylcholine 2-acylhydrolase; calcium-dependent phospholipid-binding protein
UniProt Protein Name
Cytosolic phospholipase A2
UniProt Gene Name
PLA2G4A
UniProt Synonym Gene Names
CPLA2; PLA2G4; cPLA2
UniProt Entry Name
PA24A_HUMAN
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Product Notes
The PLA2G4A pla2g4a (Catalog #AAA45409) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospholipase A2 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospholipase A2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PLA2G4A pla2g4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospholipase A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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