Human Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1) ELISA Kit | Rock1 elisa kit

Human Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA152482

• Gene Names: ROCK1; ROCK-I; P160ROCK
• Reactivity: Human
• Synonyms: Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1); N/A; Human Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1)(Sandwich ELISA) ELISA Kit; ELISA Kit for Rho Associated Coiled Coil Containing Protein Kinase 1 (Rock1); p160ROCK; Rho-associated protein kinase 1; Renal carcinoma antigen NY-REN-35; Rock1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rock1. No significant cross-reactivity or interference between Rock1 and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rock1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rock1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life); Shelf-life: one year; The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for Rock1 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Rock1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Rock1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rock1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rock1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Rock1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 47605999
• NCBI GeneID: 6093
• UniProt Accession #: Q13464; Q2KHM4; Q59GZ4; B0YJ91
• Molecular Weight: 1354
• NCBI Official Full Name: Rho-associated protein kinase 1
• NCBI Official Synonym Full Names: Rho-associated, coiled-coil containing protein kinase 1
• NCBI Official Symbol: ROCK1
• NCBI Official Synonym Symbols: ROCK-I; P160ROCK
• NCBI Protein Information: rho-associated protein kinase 1; p160-ROCK; Rho kinase; p160 ROCK-1; renal carcinoma antigen NY-REN-35; rho-associated, coiled-coil-containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase I
• UniProt Protein Name: Rho-associated protein kinase 1
• UniProt Gene Name: ROCK1
• UniProt Synonym Gene Names: ROCK-I; p160ROCK
• UniProt Entry Name: ROCK1_HUMAN