Standard Curve (Sample)
(Fig.1. Human Solute carrier family 15 member 2 (SLC15A2) Standard Curve.)
Human Solute Carrier Family 15 Member 2 (SLC15A2) ELISA Kit | SLC15A2 elisa kit
Human Solute Carrier Family 15 Member 2 (SLC15A2) ELISA Kit
Gene Names
SLC15A2; PEPT2
Reactivity
Human
Synonyms
Solute Carrier Family 15 Member 2 (SLC15A2); N/A; Human Solute Carrier Family 15 Member 2 (SLC15A2) ELISA Kit; SLC15A2; FLJ33407; PEPT2; kidney H(+)/peptide cotransporter; oligopeptide transporter; kidney isoform; peptide transporter 2; SLC15A2 elisa kit
Reactivity
Human
Specificity
Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC15A2. No significant cross-reactivity or interference between Human SLC15A2 and analogues was observed.
Sequence Length
698
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Solute carrier family 15 member 2 (SLC15A2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Solute carrier family 15 member 2 (SLC15A2) Standard Curve.)
Related Product Information for SLC15A2 elisa kit
Description: This Human Solute carrier family 15 member 2 (SLC15A2) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC15A2 in samples. An antibody specific for SLC15A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC15A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC15A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC15A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: This transporter, SLC15A2, belongs to the same gene family as SLC15A1, the proton-coupled peptide transporter found in the small intestine.The SLC15A2 cDNA encodes a predicted 729-amino acid protein that contains 12 transmembrane domains and a long hydrophilic segment between transmembrane domains 9 and 10. The amino acid sequence of SLC15A2 shows 50% identity and 70% similarity with that of SLC15A1. Functional expression of the SLC15A2 cDNA in HeLa cells resulted in the induction of a proton-dependent transport system that was able to transport dipeptides, tripeptides, and beta-lactam antibiotics; free amino acids were not accepted as substrates. RT-PCR showed that SLC15A2 is expressed in kidney but not in intestine.
Background: This transporter, SLC15A2, belongs to the same gene family as SLC15A1, the proton-coupled peptide transporter found in the small intestine.The SLC15A2 cDNA encodes a predicted 729-amino acid protein that contains 12 transmembrane domains and a long hydrophilic segment between transmembrane domains 9 and 10. The amino acid sequence of SLC15A2 shows 50% identity and 70% similarity with that of SLC15A1. Functional expression of the SLC15A2 cDNA in HeLa cells resulted in the induction of a proton-dependent transport system that was able to transport dipeptides, tripeptides, and beta-lactam antibiotics; free amino acids were not accepted as substrates. RT-PCR showed that SLC15A2 is expressed in kidney but not in intestine.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
solute carrier family 15 member 2 isoform b
NCBI Official Synonym Full Names
solute carrier family 15 member 2
NCBI Official Symbol
SLC15A2
NCBI Official Synonym Symbols
PEPT2
NCBI Protein Information
solute carrier family 15 member 2
UniProt Protein Name
Solute carrier family 15 member 2
UniProt Gene Name
SLC15A2
UniProt Synonym Gene Names
PEPT2
UniProt Entry Name
S15A2_HUMAN
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Product Notes
The Human SLC15A2 slc15a2 (Catalog #AAA330837) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330837 ELISA Kit recognizes Human SLC15A2. It is sometimes possible for the material contained within the vial of "Solute Carrier Family 15 Member 2 (SLC15A2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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