Standard Curve (Sample)
(Fig.1. Human Tau-tubulin kinase 2 (TTBK2) Standard Curve.)
Human Tau-Tubulin Kinase 2 (TTBK2) ELISA Kit | TTBK2 elisa kit
Human Tau-Tubulin Kinase 2 (TTBK2) ELISA Kit
Gene Names
TTBK2; TTBK; SCA11
Reactivity
Human
Synonyms
Tau-Tubulin Kinase 2 (TTBK2); N/A; Human Tau-Tubulin Kinase 2 (TTBK2) ELISA Kit; TTBK2; KIAA0847; SCA11; TTBK; spinocerebellar ataxia 11; TTBK2 elisa kit
Reactivity
Human
Specificity
Human Tau-tubulin kinase 2 (TTBK2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TTBK2. No significant cross-reactivity or interference between Human TTBK2 and analogues was observed.
Sequence Length
1244
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Tau-tubulin kinase 2 (TTBK2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Tau-tubulin kinase 2 (TTBK2) Standard Curve.)
Related Product Information for TTBK2 elisa kit
Description: This Human Tau-tubulin kinase 2 (TTBK2) ELISA Kit employs a two-site sandwich ELISA to quantitate TTBK2 in samples. An antibody specific for TTBK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TTBK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTBK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTBK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TTBK2 produces a 5.6-kb transcript in which the longest open reading frame is 3,732 nucleotides, encoding a protein of 1,244 amino acids. The gene is alternatively spliced, with ubiquitous expression in human adult and fetal tissues. The N-terminus of TTBK2 encodes a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK2 was expressed in all brain regions in human, rat, and mouse tissue. There was particularly high expression in Purkinje cells of the cerebellum, granular cell layer, hippocampus, midbrain, and substantia nigra. Lower expression was seen in the cortex of human, rat, and mouse brains.2 TTBK2 phosphorylation sites in tau (ser208 and ser210) are priming sites for the phosphorylation of tau by GSK-3-beta, which influences tau pathology.
Background: TTBK2 produces a 5.6-kb transcript in which the longest open reading frame is 3,732 nucleotides, encoding a protein of 1,244 amino acids. The gene is alternatively spliced, with ubiquitous expression in human adult and fetal tissues. The N-terminus of TTBK2 encodes a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK2 was expressed in all brain regions in human, rat, and mouse tissue. There was particularly high expression in Purkinje cells of the cerebellum, granular cell layer, hippocampus, midbrain, and substantia nigra. Lower expression was seen in the cortex of human, rat, and mouse brains.2 TTBK2 phosphorylation sites in tau (ser208 and ser210) are priming sites for the phosphorylation of tau by GSK-3-beta, which influences tau pathology.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
TTBK2 protein
NCBI Official Synonym Full Names
tau tubulin kinase 2
NCBI Official Symbol
TTBK2
NCBI Official Synonym Symbols
TTBK; SCA11
NCBI Protein Information
tau-tubulin kinase 2
UniProt Protein Name
Tau-tubulin kinase 2
UniProt Gene Name
TTBK2
UniProt Synonym Gene Names
KIAA0847
UniProt Entry Name
TTBK2_HUMAN
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Product Notes
The Human TTBK2 ttbk2 (Catalog #AAA330344) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330344 ELISA Kit recognizes Human TTBK2. It is sometimes possible for the material contained within the vial of "Tau-Tubulin Kinase 2 (TTBK2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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