Rabbit TTBK2 Polyclonal Antibody | anti-TTBK2 antibody

TTBK2 Antibody (N-term)

Cat. Num. AAA286485

• Gene Names: TTBK2; TTBK; SCA11
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TTBK2, Antibody; TTBK2 Antibody (N-term); Tau-tubulin kinase 2; TTBK2; KIAA0847; anti-TTBK2 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TTBK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-245 amino acids from the N-terminal region of human TTBK2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 217-245
• Sequence Length: 1244

• Applicable Applications for anti-TTBK2 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Function: Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.
• Cellular Location: Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytosol. Nucleus. Note=Localizes to the transition zone in primary cilia in response to cell cycle signals that promote ciliogenesis (By similarity). May also be present in cytosol and, at lower level in the nucleus.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(TTBK2 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(TTBK2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TTBK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(TTBK2 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TTBK2 antibody detected the TTBK2 protein (arrow).)

Related Product Information for anti-TTBK2 antibody

This gene encodes a serine-threonine kinase that
putatively phosphorylates tau and tubulin proteins. Mutations in
this gene cause spinocerebellar ataxia type 11 (SCA11); a
neurodegenerative disease characterized by progressive ataxia and
atrophy of the cerebellum and brainstem.

Product Categories/Family for anti-TTBK2 antibody

Neuroscience; Signal Transduction

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Xu, Q., et al. Neurol. Sci. 31(1):107-109(2010)
Edener, U., et al. J. Neurol. 256(11):1856-1859(2009)
Houlden, H., et al. Nat. Genet. 39(12):1434-1436(2007)
Kitano-Takahashi, M., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63 (PT 7), 602-604 (2007) :

NCBI and Uniprot Product Information

• NCBI GI #: 116812624
• NCBI GeneID: 146057
• NCBI Accession #: NP_775771.3
• NCBI GenBank Nucleotide #: NM_173500.3
• UniProt Accession #: Q6IQ55; O94932; Q6ZN52; Q8IVV1
• Molecular Weight: 137412
• NCBI Official Full Name: tau-tubulin kinase 2
• NCBI Official Synonym Full Names: tau tubulin kinase 2
• NCBI Official Symbol: TTBK2
• NCBI Official Synonym Symbols: TTBK; SCA11
• NCBI Protein Information: tau-tubulin kinase 2
• UniProt Protein Name: Tau-tubulin kinase 2
• UniProt Gene Name: TTBK2
• UniProt Synonym Gene Names: KIAA0847
• UniProt Entry Name: TTBK2_HUMAN