Standard Curve (Sample)
(Fig.1. Human Thymidylate synthase (TYMS) Standard Curve.)
Human Thymidylate Synthase (TYMS) ELISA Kit | TYMS elisa kit
Human Thymidylate Synthase (TYMS) ELISA Kit
Gene Names
TYMS; TS; TMS; HST422
Reactivity
Human
Synonyms
Thymidylate Synthase (TYMS); N/A; Human Thymidylate Synthase (TYMS) ELISA Kit; TYMS; HST422; MGC88736; TMS; TS; TSase; thymidylate synthase; TYMS elisa kit
Reactivity
Human
Specificity
Human Thymidylate synthase (TYMS) ELISA Kit has high sensitivity and excellent specificity for detection of Human TYMS. No significant cross-reactivity or interference between Human TYMS and analogues was observed.
Sequence Length
313
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Thymidylate synthase (TYMS) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Thymidylate synthase (TYMS) Standard Curve.)
Related Product Information for TYMS elisa kit
Description: This Human Thymidylate synthase (TYMS) ELISA Kit employs a two-site sandwich ELISA to quantitate TYMS in samples. An antibody specific for TYMS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TYMS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TYMS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TYMS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Thymidylate synthetase (EC 2.1.1.45) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product. As an anti-cancer chemotherapy target, thymidylate synthetase can be inhibited by the thymidylate synthase inhibitors such as fluorinated pyrimidine fluorouracil, or certain folate analogues, including most notably raltitrexed (trade name Tomudex).
Background: Thymidylate synthetase (EC 2.1.1.45) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. By means of reductive methylation, deoxyuridine monophosphate (dUMP) and N5,N10-methylene tetrahydrofolate are together used to form dTMP, yielding dihydrofolate as a secondary product. As an anti-cancer chemotherapy target, thymidylate synthetase can be inhibited by the thymidylate synthase inhibitors such as fluorinated pyrimidine fluorouracil, or certain folate analogues, including most notably raltitrexed (trade name Tomudex).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Thymidylate synthase
NCBI Official Synonym Full Names
thymidylate synthetase
NCBI Official Symbol
TYMS
NCBI Official Synonym Symbols
TS; TMS; HST422
NCBI Protein Information
thymidylate synthase
UniProt Protein Name
Thymidylate synthase
UniProt Gene Name
TYMS
UniProt Synonym Gene Names
TS; TS; TSase
UniProt Entry Name
TYSY_HUMAN
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Product Notes
The Human TYMS tyms (Catalog #AAA330333) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330333 ELISA Kit recognizes Human TYMS. It is sometimes possible for the material contained within the vial of "Thymidylate Synthase (TYMS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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