Mouse Thymidylate Synthase Monoclonal Antibody | anti-TYMS antibody

Thymidylate Synthase (5-FU Resistance Marker)

Cat. Num. AAA214828

• Gene Names: TYMS; TS; TMS; HST422
• Reactivity: Human. Others not known.
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Synonyms: Thymidylate Synthase, Antibody; Thymidylate Synthase (5-FU Resistance Marker); dTMP synthase; TMS; TS; TSase; TYMS protein; Tyms thymidylate synthetase; anti-TYMS antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG2c, kappa
• Clone Number: TYMS/1884
• Specificity: It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA at 1.0mg/ml
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
• Sequence Length: 279

• Applicable Applications for anti-TYMS antibody: IHC (Immunohistochemistry)
• Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
• Positive Control: 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1); 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.
• Cellular Localization: Nuclear & Cytoplasmic
• Chromosome Location: 18p11.32
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteins using Thymidylate Synthase (TYMS) Mouse Monoclonal Antibody (TYMS/1884). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

WB (Western Blot)

(Western Blot Analysis of human HeLa Cell Lysate using Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).)

SDS-PAGE

(SDS-PAGE Analysis Purified Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Thymidylate Synthase Mouse Monoclonal Antibody (TYMS/1884).)

References

Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13.; Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31.

NCBI and Uniprot Product Information

• NCBI GI #: 4507751
• NCBI GeneID: 7298
• NCBI Accession #: NP_001062.1
• NCBI GenBank Nucleotide #: NM_001071.3
• UniProt Accession #: P04818; Q8WYK3; Q8WYK4
• Molecular Weight: 36kDa
• NCBI Official Full Name: thymidylate synthase isoform 1
• NCBI Official Synonym Full Names: thymidylate synthetase
• NCBI Official Symbol: TYMS
• NCBI Official Synonym Symbols: TS; TMS; HST422
• NCBI Protein Information: thymidylate synthase
• UniProt Protein Name: Thymidylate synthase
• UniProt Gene Name: TYMS
• UniProt Synonym Gene Names: TS; TS; TSase