Mouse anti-Human Thymidylate Synthase Antibody | anti-TYMS antibody

Thymidylate Synthase (5-FU Resistance Marker)

Cat. Num. AAA215240

• Gene Names: TYMS; TS; TMS; HST422
• Reactivity: Human
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Thymidylate Synthase; N/A; Thymidylate Synthase (5-FU Resistance Marker); dTMP synthase; TMS; TS; TSase; TYMS protein; Tyms thymidylate synthetase; anti-TYMS antibody

• Host: Mouse
• Reactivity: Human
• Isotype: IgG1, kappa
• Clone Number: rTYMS/1884
• Specificity: It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 1.0mg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05%.
• Sequence Length: 313

• Applicable Applications for anti-TYMS antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
• Positive Control: MOLT4; Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1); 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.
• Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174) (exact sequence is proprietary)
• Cellular Localization: Nuclear & Cytoplasmic
• Hu-chromosome Location: 18p11.32
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C. Non-hazardous. No MSDS required.

IF (Immunofluorescence)

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed MOLT4 cells. TYMS Recombinant Mouse Monoclonal Antibody (rTYMS/1884) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

SDS-PAGE

(SDS-PAGE Analysis Purified TYMS Recombinant Mouse Monoclonal Antibody (rTYMS/1884). Confirmation of Purity and Integrity of Antibody.)

References

1. Johnston PG, et. al. Journal of the National Cancer Institute, 1997, 89(4):308-13. 2. Pestalozzi BC, et. al. Journal of Clinical Oncology, 1997, 15(5):1923-31

NCBI and Uniprot Product Information

• NCBI GI #: 136611
• NCBI GeneID: 7298
• UniProt Accession #: P04818
• Molecular Weight: 36kDa
• NCBI Official Full Name: Thymidylate synthase
• NCBI Official Synonym Full Names: thymidylate synthetase
• NCBI Official Symbol: TYMS
• NCBI Official Synonym Symbols: TS; TMS; HST422
• NCBI Protein Information: thymidylate synthase
• UniProt Protein Name: Thymidylate synthase
• UniProt Gene Name: TYMS
• UniProt Synonym Gene Names: TS; TS; TSase
• UniProt Entry Name: TYSY_HUMAN