Standard Curve (Sample)
(Human TLR3 PicoKine ELISA Kit standard curve)
Human TLR3 ELISA Kit | TLR3 elisa kit
Human TLR3 PicoKine ELISA Kit
Gene Names
TLR3; CD283; IIAE2
Reactivity
Human
Synonyms
TLR3; N/A; Human TLR3 PicoKine ELISA Kit; Toll-like receptor 3; toll-like receptor 3; CD_antigen=CD283; CD283; IIAE2; Toll like receptor 3; TLR3 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human TLR3
Sequence Length
627
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Human TLR3 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human TLR3 PicoKine ELISA Kit standard curve)
Related Product Information for TLR3 elisa kit
Principle of the Assay: The human TLR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TLR3 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: K27-S711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TLR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TLR3 amount of sample captured in plate.
Background: Toll-like receptor 3 (TLR3) also known as C0283 (cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA (dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.
Background: Toll-like receptor 3 (TLR3) also known as C0283 (cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA (dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
72,937 Da
NCBI Official Synonym Full Names
toll-like receptor 3
NCBI Official Symbol
TLR3
NCBI Official Synonym Symbols
CD283; IIAE2
NCBI Protein Information
toll-like receptor 3
UniProt Protein Name
Toll-like receptor 3
UniProt Gene Name
TLR3
UniProt Entry Name
TLR3_HUMAN
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Product Notes
The Human TLR3 tlr3 (Catalog #AAA45203) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45203 ELISA Kit recognizes Human TLR3. It is sometimes possible for the material contained within the vial of "TLR3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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