Human Total MMP-1 ELISA Kit | MMP-1 elisa kit

Total MMP-1 (Human) OmniKine ELISA Kit

Cat. Num. AAA315757

• Gene Names: MMP1; CLG; CLGN
• Reactivity: Human
• Synonyms: Total MMP-1; N/A; Total MMP-1 (Human) OmniKine ELISA Kit; Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; CLG; MMP-1 elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human MMP-1 ELISA is capable of recognizing both recombinant and naturally produced Human MMP-1 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 469
• Assay Type: Quantitative Sandwich
• Samples: Cell Lysates, Sera and Plasma
• Sensitivity: 157-10000 pg/ml

• Detection Method: Colorimetric 450 nm
• Subtype: None
• Preparation and Storage: Store entire kit at 4 degree C until use.

Standard Curve (Sample)

Related Product Information for MMP-1 elisa kit

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.. Alternatively spliced transcript variants encoding different isoforms have been identified. Human total MMP-1 cleaves collagens of types I, II, and III at one site in the helical domain and also cleaves collagens of types VII and X. In case of HIV infection, MMP-1 interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Cleavage of the triple helix of collagen occurs at about threequarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile- 776 in the alpha-1(I) chain. The proteinase cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.There are two distinct domains in this protein: the catalytic Nterminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases). The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. MMP-1 undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

Principle of the Assay: The OmniKine™ Human Total MMP-1 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Total MMP-1 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" EnzymeLinked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Total MMP-1 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

NCBI and Uniprot Product Information

• NCBI GI #: 4505215
• NCBI GeneID: 4312
• NCBI Accession #: NP_002412.1
• NCBI GenBank Nucleotide #: NM_002421.3
• UniProt Accession #: P03956; P08156
• Molecular Weight: 54,007 Da
• NCBI Official Full Name: interstitial collagenase isoform 1 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 1
• NCBI Official Symbol: MMP1
• NCBI Official Synonym Symbols: CLG; CLGN
• NCBI Protein Information: interstitial collagenase
• UniProt Protein Name: Interstitial collagenase
• UniProt Gene Name: MMP1
• UniProt Synonym Gene Names: CLG; MMP-1