Standard Curve (Sample)
(Fig.1. Human TRAF family member-associated NF-kappa-B activator (TANK) Standard Curve.)
Human TRAF Family Member-Associated NF-kappa-B Activator (TANK) ELISA Kit | TANK elisa kit
Human TRAF Family Member-Associated NF-kappa-B Activator (TANK) ELISA Kit
Gene Names
TANK; ITRAF; TRAF2; I-TRAF
Reactivity
Human
Synonyms
TRAF Family Member-Associated NF-kappa-B Activator (TANK); N/A; Human TRAF Family Member-Associated NF-kappa-B Activator (TANK) ELISA Kit; TANK; I-TRAF; TRAF2; TRAF family member-associated NF-KAPPA-B activator; TRAF interacting protein TANK; TANK elisa kit
Reactivity
Human
Specificity
Human TRAF family member-associated NF-kappa-B activator (TANK) ELISA Kit has high sensitivity and excellent specificity for detection of Human TANK. No significant cross-reactivity or interference between Human TANK and analogues was observed.
Sequence Length
425
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human TRAF family member-associated NF-kappa-B activator (TANK) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human TRAF family member-associated NF-kappa-B activator (TANK) Standard Curve.)
Related Product Information for TANK elisa kit
Description: This Human TRAF family member-associated NF-kappa-B activator (TANK) ELISA Kit employs a two-site sandwich ELISA to quantitate TANK in samples. An antibody specific for TANK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TANK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TANK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TANK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. TRAF family member-associated NF-kappa-B activator (TANK) is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by TANK can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for TANK.
Background: The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. TRAF family member-associated NF-kappa-B activator (TANK) is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by TANK can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for TANK.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
TRAF family member-associated NF-kappa-B activator isoform a
NCBI Official Synonym Full Names
TRAF family member associated NFKB activator
NCBI Official Symbol
TANK
NCBI Official Synonym Symbols
ITRAF; TRAF2; I-TRAF
NCBI Protein Information
TRAF family member-associated NF-kappa-B activator
UniProt Protein Name
TRAF family member-associated NF-kappa-B activator
UniProt Gene Name
TANK
UniProt Synonym Gene Names
ITRAF; TRAF2; I-TRAF
UniProt Entry Name
TANK_HUMAN
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Product Notes
The Human TANK tank (Catalog #AAA330681) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330681 ELISA Kit recognizes Human TANK. It is sometimes possible for the material contained within the vial of "TRAF Family Member-Associated NF-kappa-B Activator (TANK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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