Mouse TRAF Family Member-Associated NF-kappa-B Activator (TANK) ELISA Kit | TANK elisa kit

Mouse TRAF Family Member-Associated NF-kappa-B Activator (TANK) ELISA Kit

Cat. Num. AAA332345

• Gene Names: Tank; C86182; I-TRAF; E430026L09Rik
• Reactivity: Mouse
• Synonyms: TRAF Family Member-Associated NF-kappa-B Activator (TANK); N/A; Mouse TRAF Family Member-Associated NF-kappa-B Activator (TANK) ELISA Kit; TANK; I-TRAF; TRAF2; TRAF family member-associated NF-KAPPA-B activator; TRAF interacting protein TANK; TANK elisa kit

• Reactivity: Mouse
• Specificity: Mouse TRAF family member-associated NF-kappa-B activator (TANK) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse TANK. No significant cross-reactivity or interference between Mouse TANK and analogues was observed.
• Sequence Length: 413
• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids

• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Standard Curve (Sample)

(Fig.1. Mouse TRAF family member-associated NF-kappa-B activator (TANK) Standard Curve.)

Related Product Information for TANK elisa kit

Description: This Mouse TRAF family member-associated NF-kappa-B activator (TANK) ELISA Kit employs a two-site sandwich ELISA to quantitate TANK in samples. An antibody specific for TANK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TANK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TANK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TANK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by TANK is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by TANK can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Two transcript variants encoding different isoforms have been found for TANK.

NCBI and Uniprot Product Information

• NCBI GI #: 255759991
• NCBI GeneID: 21353
• NCBI Accession #: NP_001157544.1
• NCBI GenBank Nucleotide #: NP_001157544.1
• UniProt Accession #: P70347
• NCBI Official Full Name: TRAF family member-associated NF-kappa-B activator isoform 2
• NCBI Official Synonym Full Names: TRAF family member-associated Nf-kappa B activator
• NCBI Official Symbol: Tank
• NCBI Official Synonym Symbols: C86182; I-TRAF; E430026L09Rik
• NCBI Protein Information: TRAF family member-associated NF-kappa-B activator
• UniProt Protein Name: TRAF family member-associated NF-kappa-B activator
• UniProt Gene Name: Tank
• UniProt Synonym Gene Names: Itraf; I-TRAF
• UniProt Entry Name: TANK_MOUSE