Human Transcription Factor Binding To IGHM Enhancer 3 (TFE3) ELISA Kit | TFE3 elisa kit

Human Transcription Factor Binding To IGHM Enhancer 3 (TFE3) ELISA Kit

Cat. Num. AAA129551

• Gene Names: TFE3; TFEA; RCCP2; RCCX1; bHLHe33
• Reactivity: Human
• Synonyms: Transcription Factor Binding To IGHM Enhancer 3 (TFE3); N/A; Human Transcription Factor Binding To IGHM Enhancer 3 (TFE3) ELISA Kit; RCCP2; TFEA; BHLHE33; Transcription Tactor E Family Member A; Class E basic helix-loop-helix protein 33; Transcription factor E3; TFE3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transcription Factor Binding To IGHM Enhancer 3 (TFE3).; No significant cross-reactivity or interference between Transcription Factor Binding To IGHM Enhancer 3 (TFE3) and analogues was observed.
• Sequence Length: 575
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.114ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor Binding To IGHM Enhancer 3 (TFE3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor Binding To IGHM Enhancer 3 (TFE3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for TFE3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor Binding To IGHM Enhancer 3 (TFE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcription Factor Binding To IGHM Enhancer 3 (TFE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TFE3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 19684176
• NCBI GeneID: 7030
• UniProt Accession #: P19532; Q5JU74; Q92757; Q92758; Q99964; A8MZL6
• Molecular Weight: 11,363 Da
• NCBI Official Full Name: Transcription factor binding to IGHM enhancer 3
• NCBI Official Synonym Full Names: transcription factor binding to IGHM enhancer 3
• NCBI Official Symbol: TFE3
• NCBI Official Synonym Symbols: TFEA; RCCP2; RCCX1; bHLHe33
• NCBI Protein Information: transcription factor E3
• UniProt Protein Name: Transcription factor E3
• UniProt Gene Name: TFE3
• UniProt Synonym Gene Names: BHLHE33; bHLHe33
• UniProt Entry Name: TFE3_HUMAN