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IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.)
Rabbit anti-Human, mouse TFE3 Polyclonal Antibody | anti-TFE3 antibody
TFE3 Antibody (N-term)
Gene Names
TFE3; TFEA; RCCP2; RCCX1; bHLHe33
Reactivity
Human, mouse
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
TFE3, Antibody; TFE3 Antibody (N-term); Transcription factor E3; Class E basic helix-loop-helix protein 33; bHLHe33; TFE3; BHLHE33; anti-TFE3 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This TFE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TFE3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
1-30
Sequence Length
575
Applicable Applications for anti-TFE3 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Clone Names
RB20263
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with TFE3 Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFE3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TFE3 immunoreactivity is localized to nucleus and Cytoplasm significantly.)
WB (Western Blot)
(TFE3 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).)
WB (Western Blot)
(TFE3 Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the TFE3 antibody detected the TFE3 protein (arrow).)
WB (Western Blot)
(TFE3 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).)
WB (Western Blot)
(TFE3 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TFE3 Antibody detected the TFE3 protein (arrow).)
WB (Western Blot)
(Anti-TFE3 Antibody (N-term)at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-TFE3 Antibody (N-term)at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-TFE3 antibody
The microphthalmia transcription factor/transcription
factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper
(bHLH-Zip) transcription factors includes four family members:
MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene
activates transcription through binding to the muE3 motif of the
immunoglobulin heavy-chain enhancer. The TFEC protein forms
heterodimers with the TEF3 protein and inhibits TFE3-dependent
transcription activation. The TEF3 protein interacts with
transcription regulators such as E2F3, SMAD3, and LEF-1, and is
involved in TGF-beta-induced transcription, playing important roles
in cell growth, proliferation, and osteoclast and macrophage
differentiation. The TFE3 protein also activates hepatic IRS-2
gene, and induces hexokinase II (HK2) and insulin-induced gene 1
(INSIG1); it participates in insulin signaling and could be a
therapeutic target for diabetes. This gene is also involved in
chromosomal translocations, resulting in different fusion gene
products in papillary renal cell carcinomas and alveolar soft part
sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO
(p54nrb)-TFE3 and ASPL-TFE3.
factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper
(bHLH-Zip) transcription factors includes four family members:
MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene
activates transcription through binding to the muE3 motif of the
immunoglobulin heavy-chain enhancer. The TFEC protein forms
heterodimers with the TEF3 protein and inhibits TFE3-dependent
transcription activation. The TEF3 protein interacts with
transcription regulators such as E2F3, SMAD3, and LEF-1, and is
involved in TGF-beta-induced transcription, playing important roles
in cell growth, proliferation, and osteoclast and macrophage
differentiation. The TFE3 protein also activates hepatic IRS-2
gene, and induces hexokinase II (HK2) and insulin-induced gene 1
(INSIG1); it participates in insulin signaling and could be a
therapeutic target for diabetes. This gene is also involved in
chromosomal translocations, resulting in different fusion gene
products in papillary renal cell carcinomas and alveolar soft part
sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO
(p54nrb)-TFE3 and ASPL-TFE3.
Product Categories/Family for anti-TFE3 antibody
References
Argani, P., et al. Am. J. Surg. Pathol. 34(10):1395-1406(2010)
Haudebourg, J., et al. Cancer Genet. Cytogenet. 200(2):75-78(2010)
Chang, I.W., et al. Am. J. Surg. Pathol. 33(12):1894-1901(2009)
Yamaguchi, T., et al. Acta Cytol. 53(6):693-697(2009)
Kuroda, N., et al. Pathol. Int. 59(10):769-770(2009)
Haudebourg, J., et al. Cancer Genet. Cytogenet. 200(2):75-78(2010)
Chang, I.W., et al. Am. J. Surg. Pathol. 33(12):1894-1901(2009)
Yamaguchi, T., et al. Acta Cytol. 53(6):693-697(2009)
Kuroda, N., et al. Pathol. Int. 59(10):769-770(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61521
NCBI Official Full Name
transcription factor E3 isoform 1
NCBI Official Synonym Full Names
transcription factor binding to IGHM enhancer 3
NCBI Official Symbol
TFE3
NCBI Official Synonym Symbols
TFEA; RCCP2; RCCX1; bHLHe33
NCBI Protein Information
transcription factor E3
UniProt Protein Name
Transcription factor E3
UniProt Gene Name
TFE3
UniProt Synonym Gene Names
BHLHE33; bHLHe33
UniProt Entry Name
TFE3_HUMAN
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Product Notes
The TFE3 tfe3 (Catalog #AAA288817) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The TFE3 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TFE3 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TFE3 tfe3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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