Application Data
(AbFlex CTCF recombinant antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 250,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NextSeq 500/550. Data was collected from 21 million reads, and CTCF data is shown for Chromosome 1.)
Rabbit anti-Human CTCF Antibody | anti-CTCF antibody
AbFlex CTCF antibody (rAb)
Gene Names
CTCF; MRD21
Reactivity
Human
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
CTCF; N/A; AbFlex CTCF antibody (rAb); CCCTC-Binding Factor; MRD21; anti-CTCF antibody
Host
Rabbit
Reactivity
Human
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in 140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-CTCF antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation)
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against a peptide within human CTCF.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Application Data
(AbFlex CTCF recombinant antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 250,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NextSeq 500/550. Data was collected from 21 million reads, and CTCF data is shown for Chromosome 1.)
Application Data
(AbFlex CTCF recombinant antibody (rAb) tested by CUT&Tag CUT&Tag was performed using 250,000 K562 cells and sequenced using 38 base-pair, paired-end reads on the Illumina NextSeq 500/550. Data was collected from 21 million reads, and CTCF data is shown for Chromosome 1.)
WB (Western Blot)
(AbFlex CTCF antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex CTCF antibody at 0.2 ug/ml.)
WB (Western Blot)
(AbFlex CTCF antibody (rAb) tested by Western blot. 20 ug of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex CTCF antibody at 0.2 ug/ml.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex CTCF recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of MCF7 chromatin and 4 ug of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to identify CTCF binding sites on chromosome 9.)
ChIP (Chromatin Immunoprecipitation)
(AbFlex CTCF recombinant antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of MCF7 chromatin and 4 ug of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 8.9 million sequence tags were mapped to identify CTCF binding sites on chromosome 9.)
Related Product Information for anti-CTCF antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex CTCF antibody (rAb) was raised in a Rabbit host. It has been validated for use in ChIP-Seq, CUT&Tag and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex CTCF antibody was expressed in CHO cells and contains rabbit immunoglobulin heavy and light chains. CTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. It is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex CTCF antibody was expressed in CHO cells and contains rabbit immunoglobulin heavy and light chains. CTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. It is involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping.
Product Categories/Family for anti-CTCF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
83kDa
NCBI Official Full Name
transcriptional repressor CTCF isoform 1
NCBI Official Synonym Full Names
CCCTC-binding factor
NCBI Official Symbol
CTCF
NCBI Official Synonym Symbols
MRD21
NCBI Protein Information
transcriptional repressor CTCF
UniProt Protein Name
Transcriptional repressor CTCF
UniProt Gene Name
CTCF
UniProt Entry Name
CTCF_HUMAN
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Product Notes
The CTCF ctcf (Catalog #AAA60284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AbFlex CTCF antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CTCF can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the CTCF ctcf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTCF, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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