Human TRPM8 (Transient receptor potential cation channel subfamily M member 8) ELISA Kit | TRPM8 elisa kit

Human TRPM8 (Transient receptor potential cation channel subfamily M member 8) ELISA Kit

Cat. Num. AAA248791

• Gene Names: TRPM8; TRPP8; LTRPC6; trp-p8; LTrpC-6
• Reactivity: Human
• Synonyms: TRPM8 (Transient receptor potential cation channel subfamily M member 8); N/A; Human TRPM8 (Transient receptor potential cation channel subfamily M member 8) ELISA Kit; TRPM8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection ofTRPM8. No significant cross-reactivity or interference betweenTRPM8and analogues was observed.
• Sequence Length: 1104
• Samples: serum, plasma, tissue homogenatesand other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelTRPM8were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelTRPM8were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for TRPM8 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TRPM8antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TRPM8 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TRPM8 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TRPM8 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 109689695
• NCBI GeneID: 79054
• NCBI Accession #: NP_076985.4
• NCBI GenBank Nucleotide #: NM_024080.4
• UniProt Accession #: Q7Z2W7; Q3YFM7; Q6QNH9; Q8TAC3; Q8TDX8; Q9BVK1; A0AVG2
• Molecular Weight: 79,096 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 8
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 8
• NCBI Official Symbol: TRPM8
• NCBI Official Synonym Symbols: TRPP8; LTRPC6; trp-p8; LTrpC-6
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 8
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 8
• UniProt Gene Name: TRPM8
• UniProt Synonym Gene Names: LTRPC6; TRPP8; LTrpC-6; LTrpC6; Trp-p8