Rabbit TRPM8 Polyclonal Antibody | anti-TRPM8 antibody

TRPM8 (Extracellular region) Antibody

Cat. Num. AAA71723

• Gene Names: TRPM8; TRPP8; LTRPC6
• Reactivity: Human, Rat, Mouse
• Applications: Immunocytochemistry, ELISA, Western Blot
• Synonyms: TRPM8, Antibody; TRPM8 (Extracellular region) Antibody; TRPM8 (Extracellular region); TRPM8 917pAb; anti-TRPM8 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Specificity: The antibody was affinity purified using TRPM8 (Extracellular region) peptide. This antibody antibody detects a 130kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 and MCF-7 cells. The antibody is also recommended for immunocytochemical labeling of cells.
• Form/Format: Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-TRPM8 antibody: ICC (Immunocytochemistry), ELISA, WB (Western Blot)
• Immunogen: TRPM8 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM8. This site is well conserved in rat and mouse TRPM8, but has low homology to other TRPM family members.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with rabbit polyclonal anti-TRPM8 (TP5701). The antibody was detected using goat anti-rabbit DyLight 594.)

WB (Western Blot)

(Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with rabbit polyclonal anti-TRPM8 (extracellular region) without peptide blocking (lane 1) or with peptide blocking (lane 2).)

Related Product Information for anti-TRPM8 antibody

The Transient Receptor Potential Melastatin (TRPM) subfamily of cation-permeable channels is ubiquitous in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, some TRPM family members, TRPM6 and TRPM7, possess serine/threonine kinase activity and autophosphorylation. TRPM8 is thermoactivated at mildly cold temperatures (>25oC), and can also be activated by compounds that cause a cooling sensation, such as menthol and icilin. TRPM8 is expressed in trigeminal and dorsal root ganglia neurons where it confers sensitivity to cold in the somatosensory system. In vascular smooth muscle, TRPM8 may alter blood flow by constricting or enlarging blood vessels. TRPM8 is also expressed in normal prostate epithelial cells, as well as overexpressed in several primary tumors including colon, lung, skin, breast, and prostate cancers.

References

Bibaux, G. et al. (2015) Proc Natl Acad Sci. 112(26):E3345
Shah, A.A. et al. (2015) Clin Exp Rheuma. 33(4S91):S123
Asuthkar, S. et al. (2014) J Biol Chem. 290(5):2659
Potapova, T.A. et al. (2014) Bull Exp Biol Med. 157(6):757

NCBI and Uniprot Product Information

• NCBI GI #: 109689695
• NCBI GeneID: 79054
• NCBI Accession #: NP_076985.4
• NCBI GenBank Nucleotide #: NM_024080.4
• UniProt Accession #: Q7Z2W7; Q3YFM7; Q6QNH9; Q8TAC3; Q8TDX8; Q9BVK1; A0AVG2
• Molecular Weight: 79,096 Da
• NCBI Official Full Name: transient receptor potential cation channel subfamily M member 8
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily M member 8
• NCBI Official Symbol: TRPM8
• NCBI Official Synonym Symbols: TRPP8; LTRPC6
• NCBI Protein Information: transient receptor potential cation channel subfamily M member 8
• UniProt Protein Name: Transient receptor potential cation channel subfamily M member 8
• UniProt Gene Name: TRPM8
• UniProt Synonym Gene Names: LTRPC6; TRPP8; LTrpC-6; LTrpC6; Trp-p8
• UniProt Entry Name: TRPM8_HUMAN