Standard Curve (Sample)
(Human Tryptase PicoKine ELISA Kit standard curve)
Human Tryptase/TPSAB1,B2 PicoKine ELISA Kit | TPSAB1 elisa kit
Human Tryptase/TPSAB1,B2 PicoKine ELISA Kit
Gene Names
TPSAB1; TPS1; TPS2; TPSB1
Reactivity
Human
Synonyms
Tryptase/TPSAB1,B2 PicoKine; N/A; Human Tryptase/TPSAB1,B2 PicoKine ELISA Kit; Tryptase alpha/beta-1/beta-2; Tryptase-1; Tryptase1; Tryptase 1; TPS1; TPS2; TPSB1; Tryptase-2; Tryptase2; Tryptase 2; Tryptase-II; TryptaseII; Tryptase II; TPSB2; Tryptase alpha/beta-1; Tryptase beta-2; tryptase alpha/beta 1/beta 2; TPSAB1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human Tryptase/TPSAB1,B2
Sequence Length
275
Samples
Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
(Human Tryptase PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human Tryptase PicoKine ELISA Kit standard curve)
Related Product Information for TPSAB1 elisa kit
Principle of the Assay: The Human Tryptase/TPSAB1,B2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A specific for Tryptase/TPSAB1,B2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: M1-V275) and test samples are added to the wells, a biotinylated detection specific for Tryptase/TPSAB1,B2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Tryptase/TPSAB1,B2 amount of sample captured in plate.
Background/Introduction: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.
Background/Introduction: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,533 Da
NCBI Official Full Name
tryptase alpha/beta-1
NCBI Official Synonym Full Names
tryptase alpha/beta 1
NCBI Official Symbol
TPSAB1
NCBI Official Synonym Symbols
TPS1; TPS2; TPSB1
NCBI Protein Information
tryptase alpha/beta-1
UniProt Protein Name
Tryptase alpha/beta-1
UniProt Gene Name
TPSAB1
UniProt Synonym Gene Names
TPS1; TPS2; TPSB1; Tryptase-1
UniProt Entry Name
TRYB1_HUMAN
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Product Notes
The Human TPSAB1 tpsab1 (Catalog #AAA46162) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46162 ELISA Kit recognizes Human TPSAB1. It is sometimes possible for the material contained within the vial of "Tryptase/TPSAB1,B2 PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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