Standard Curve (Sample)
(Fig.1. Human UV excision repair protein RAD23 homolog B (RAD23B) Standard Curve.)
Human UV Excision Repair Protein RAD23 Homolog B (RAD23B) ELISA Kit | RAD23B elisa kit
Human UV Excision Repair Protein RAD23 Homolog B (RAD23B) ELISA Kit
Gene Names
RAD23B; P58; HR23B; HHR23B
Reactivity
Human
Synonyms
UV Excision Repair Protein RAD23 Homolog B (RAD23B); N/A; Human UV Excision Repair Protein RAD23 Homolog B (RAD23B) ELISA Kit; RAD23B; RP11-131A5.1; HHR23B; HR23B; P58; RAD23; yeast homolog of; B; UV excision repair protein RAD23 homolog B; XP-C repair complementing complex 58 kDa; XP-C repair complementing protein; RAD23B elisa kit
Reactivity
Human
Specificity
Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAD23B. No significant cross-reactivity or interference between Human RAD23B and analogues was observed.
Sequence Length
337
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human UV excision repair protein RAD23 homolog B (RAD23B) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human UV excision repair protein RAD23 homolog B (RAD23B) Standard Curve.)
Related Product Information for RAD23B elisa kit
Description: This Human UV excision repair protein RAD23 homolog B (RAD23B) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD23B in samples. An antibody specific for RAD23B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAD23B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD23B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD23B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RAD23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.
Background: RAD23B is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
UV excision repair protein RAD23 homolog B isoform 3
NCBI Official Synonym Full Names
RAD23 homolog B, nucleotide excision repair protein
NCBI Official Symbol
RAD23B
NCBI Official Synonym Symbols
P58; HR23B; HHR23B
NCBI Protein Information
UV excision repair protein RAD23 homolog B
UniProt Protein Name
UV excision repair protein RAD23 homolog B
UniProt Gene Name
RAD23B
UniProt Synonym Gene Names
HR23B; hHR23B; p58
UniProt Entry Name
RD23B_HUMAN
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Product Notes
The Human RAD23B rad23b (Catalog #AAA331169) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331169 ELISA Kit recognizes Human RAD23B. It is sometimes possible for the material contained within the vial of "UV Excision Repair Protein RAD23 Homolog B (RAD23B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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