Rabbit anti-Human RAD23B Polyclonal Antibody | anti-RAD23B antibody

RAD23B Antibody

Cat. Num. AAA230218

• Gene Names: RAD23B; P58; HR23B; HHR23B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Caprylic Acid Ammonium Sulfate Precipitation purified
• Synonyms: RAD23B, Antibody; RAD23B Antibody; UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58; RAD23B; anti-RAD23B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation purified
• Form/Format: Liquid
• Sequence Length: 337

• Applicable Applications for anti-RAD23B antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant human UV excision repair protein RAD23 homolog B protein
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-49042 / sc-49041 / sc-67225
• Preparation and Storage: Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human breast cancer using AAA230218 at dilution 1:100)

WB (Western Blot)

(Western blotAll lanes: RAD23B antibody at 2ug/mlLane 1:Jurkat cellsLane 2:293T cellsLane 3:A431 cellsLane 4:MCF-7 cellsSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 44,36 kDaObserved band size: 43 kDa)

Related Product Information for anti-RAD23B antibody

Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.

NCBI and Uniprot Product Information

• NCBI GI #: 347800665
• NCBI GeneID: 5887
• NCBI Accession #: NP_001231653.1
• NCBI GenBank Nucleotide #: NM_001244724.1
• UniProt Accession #: P54727; Q7Z5K8; Q8WUB0; B3KWK8; G5E9P0
• Molecular Weight: 35,005 Da
• NCBI Official Full Name: UV excision repair protein RAD23 homolog B isoform 3
• NCBI Official Synonym Full Names: RAD23 homolog B, nucleotide excision repair protein
• NCBI Official Symbol: RAD23B
• NCBI Official Synonym Symbols: P58; HR23B; HHR23B
• NCBI Protein Information: UV excision repair protein RAD23 homolog B
• UniProt Protein Name: UV excision repair protein RAD23 homolog B
• UniProt Gene Name: RAD23B
• UniProt Synonym Gene Names: HR23B; hHR23B; p58
• UniProt Entry Name: RD23B_HUMAN