IF (Immunofluorescence)
(Immunofluorescent analysis of RB1 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Rabbit RAD23B Polyclonal Antibody | anti-RAD23B antibody
Anti-RAD23B Antibody
Gene Names
RAD23B; P58; HR23B; HHR23B
Reactivity
Human, Mouse, Rat, Bovine, Monkey
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
RAD23B, Antibody; Anti-RAD23B Antibody; UV excision repair protein RAD23 homolog B; HR23B; hHR23B; XP-C repair-complementing complex 58 kDa protein; p58; anti-RAD23B antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Monkey
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of RAD23B protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
337
Applicable Applications for anti-RAD23B antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human RAD23B. The exact sequence is proprietary.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of RB1 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of RB1 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of RB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of RB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of RB1 expression in Jurkat (A), COLO205 (B), HuvEc (C), A431 (D), NIH3T3 (E) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of RB1 expression in Jurkat (A), COLO205 (B), HuvEc (C), A431 (D), NIH3T3 (E) whole cell lysates.)
Related Product Information for anti-RAD23B antibody
Rabbit polyclonal antibody to RAD23B
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,005 Da
NCBI Official Full Name
UV excision repair protein RAD23 homolog B isoform 3
NCBI Official Synonym Full Names
RAD23 homolog B (S. cerevisiae)
NCBI Official Symbol
RAD23B
NCBI Official Synonym Symbols
P58; HR23B; HHR23B
NCBI Protein Information
UV excision repair protein RAD23 homolog B; RAD23, yeast homolog of, B; XP-C repair complementing protein; XP-C repair complementing complex 58 kDa; XP-C repair-complementing complex 58 kDa protein
UniProt Protein Name
UV excision repair protein RAD23 homolog B
UniProt Gene Name
RAD23B
UniProt Synonym Gene Names
HR23B; hHR23B; p58
UniProt Entry Name
RD23B_HUMAN
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Product Notes
The RAD23B rad23b (Catalog #AAA104601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAD23B Antibody reacts with Human, Mouse, Rat, Bovine, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's RAD23B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RAD23B rad23b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAD23B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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