Human VE-Cadherin ELISA Kit | VE-Cadherin elisa kit

Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

Cat. Num. AAA21600

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Human
• Synonyms: VE-Cadherin; N/A; Human VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit; VE-Cadherin elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human VE-Cadherin in samples. No significant cross-reactivity or interference between Human VE-Cadherin and analogues was observed.
• Sequence Length: 784
• Assay Type: Quantitative Sandwich
• Samples: Serum and plasma, please inquire if your samples are other biological fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 18.75pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human VE-Cadherin were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human VE-Cadherin were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Standard Curve (Sample)

Related Product Information for VE-Cadherin elisa kit

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human VE-Cadherin. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human VE-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human VECadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human VECadherin. You can calculate the concentration of Human VE-Cadherin in the samples by comparing the OD of the samples to the standard curve.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human VE-Cadherin concentrations in serum and plasma, please inquire if your samples are other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 599834
• NCBI GeneID: 1003
• UniProt Accession #: P33151; Q4VAI5; Q4VAI6
• Molecular Weight: 74,499 Da
• NCBI Official Full Name: VE-cadherin
• NCBI Official Synonym Full Names: cadherin 5, type 2 (vascular endothelium)
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5; 7B4 antigen; VE-cadherin; cadherin 5, type 2, VE-cadherin (vascular epithelium); cd144 antigen; endothelial-specific cadherin; vascular endothelial cadherin
• UniProt Protein Name: Cadherin-5
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_HUMAN