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IHC (Immunohistochemistry)
(AAA327366 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ORAI1 Antibody | anti-ORAI1 antibody
ORAI1 Antibody
Gene Names
ORAI1; IMD9; TAM2; ORAT1; CRACM1; TMEM142A
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
ORAI1; N/A; ORAI1 Antibody; Calcium release activated calcium channel protein 1; Calcium release activated calcium modulator 1; Calcium release-activated calcium channel protein 1; CRACM 1; CRACM1; CRCM1; CRCM1_HUMAN; FLJ14466; Orai 1; ORAI calcium release activated calcium modulator 1; orai1; ORAT1; Protein orai 1; Protein orai-1; TMEM 142A; TMEM142A; Transmembrane protein 142A; anti-ORAI1 antibody
Reactivity
Human, Mouse, Rat
Specificity
ORAI1 Antibody detects endogenous levels of total ORAI1.
Purity/Purification
Peptide affinity purification
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ORAI1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ORAI1, corresponding to a region within C-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IHC (Immunohistochemistry)
(AAA327366 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA327366 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using ORAI1 Antibody. Lane 1: Rat lung, treated with blocking peptideLane 2: Rat lungLane 3: HepG2 cellsLane 4: Mcf7.Observed bands: 60 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using ORAI1 Antibody. Lane 1: Rat lung, treated with blocking peptideLane 2: Rat lungLane 3: HepG2 cellsLane 4: Mcf7.Observed bands: 60 kDa.)
WB (Western Blot)
(Western blot analysis of ORAI1 using K562 whole lysates.)
WB (Western Blot)
(Western blot analysis of ORAI1 using K562 whole lysates.)
IHC (Immunohistochemistry)
(AAA327366 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry)
(AAA327366 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-ORAI1 antibody
Ca2+ release-activated Ca2+ (CRAC) channel subunit which mediates Ca2+ influx following depletion of intracellular Ca2+ stores and channel activation by the Ca2+ sensor, STIM1 (PubMed:16582901, PubMed:16645049, PubMed:16733527, PubMed:16766533, PubMed:16807233, PubMed:19249086, PubMed:23307288, PubMed:24351972, PubMed:24591628, PubMed:28219928). CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT (PubMed:16582901)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50 kDa, 60kDa
NCBI Official Full Name
calcium release-activated calcium channel protein 1
NCBI Official Synonym Full Names
ORAI calcium release-activated calcium modulator 1
NCBI Official Symbol
ORAI1
NCBI Official Synonym Symbols
IMD9; TAM2; ORAT1; CRACM1; TMEM142A
NCBI Protein Information
calcium release-activated calcium channel protein 1
UniProt Protein Name
Calcium release-activated calcium channel protein 1
UniProt Gene Name
ORAI1
UniProt Synonym Gene Names
CRACM1; TMEM142A
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Product Notes
The ORAI1 orai1 (Catalog #AAA327366) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ORAI1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ORAI1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ORAI1 orai1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORAI1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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