WB (Western Blot)
(Western blot using anti-CD14 antibody MiL2 . U937 cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the mouse version of MiL2 (AAA72321) at 1ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
anti-Human, Pig CD14 Antibody | anti-CD14 antibody
Anti-CD14 [MiL2]
Reactivity
Human, Pig
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot
Purity
Purified. Protein A affinity purified
Synonyms
CD14; N/A; Anti-CD14 [MiL2]; Monocyte antigen; Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; anti-CD14 antibody
Reactivity
Human, Pig
Isotype
Mouse IgG2b kappa
Clone Number
MiL2
Specificity
This antibody is specific for porcine CD14.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD14 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot)
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western blot using anti-CD14 antibody MiL2 . U937 cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the mouse version of MiL2 (AAA72321) at 1ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-CD14 antibody MiL2 . U937 cells lysate (35ug protein in RIPA buffer) was resolved via SDS-PAGE, and the subsequent blot was probed with the mouse version of MiL2 (AAA72321) at 1ug/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of U937 cells with anti-CD14 antibody MiL2 . Immunofluorescence analysis of paraformaldehyde-fixed U937 cells on Shi-fix™ coverslips stained with the mouse IgG2b version of MiL2 (AAA72321) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72321, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of U937 cells with anti-CD14 antibody MiL2 . Immunofluorescence analysis of paraformaldehyde-fixed U937 cells on Shi-fix™ coverslips stained with the mouse IgG2b version of MiL2 (AAA72321) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72321, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-CD14 antibody
The original antibody was raised by immunizing a mouse with porcine CD14.
The levels of CD14 expression and LPS-binding protein (LBP) in the lungs of pigs throughout a PRRSV infection were quantified.
The levels of CD14 expression and LPS-binding protein (LBP) in the lungs of pigs throughout a PRRSV infection were quantified.
Product Categories/Family for anti-CD14 antibody
References
Gucht et al.
Viral Immunol. 2005;18(1):116-26.
Viral Immunol. 2005;18(1):116-26.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53kDa
NCBI Official Full Name
monocyte differentiation antigen CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Gene Name
CD14
UniProt Entry Name
CD14_HUMAN
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Product Notes
The CD14 cd14 (Catalog #AAA72321) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD14 [MiL2] reacts with Human, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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