Application Data
CD278 protein
CD278(ICOS)-muIg-Biotin
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Synonyms
CD278; N/A; CD278(ICOS)-muIg-Biotin; Human CD278(ICOS)-muIg/Biotin Fusion Protein; CD278 protein
Reactivity
Human
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA,0.04 NaN3 as a preservative).
Concentration
0.5 mg/ml (varies by lot)
Applicable Applications for CD278 protein
ELISA, FCM/FACS (Flow Cytometry)
Molecular Structure
A soluble molecule consisting of the extracellular (121aa) domain of mature human ICOS fused to the murine IgG2a Fc (232 aa). Predicted monomeric weight is 42.8 kd (amino acid composition only).
Transfectant Cell Line
CHO
Performance
CD278(ICOS)-mulg/Biotin was tested for its ability to bind to cell surface CD275(ICOSL) in FACS. Five x 10^5 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ul 1 of ICOS-mulg/Biotin at a concentration of 5 )Ug/ml. Cells were washed twice and incubated with 2° reagent Streptavidin/R-Phycoerythrin (Catalog #MBS666773), after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.39 log 10 fluorescent units when compared to a recombinant mulgFc Control Protein/Biotin negative control (Catalog #MBS666830). Binding was blocked when reagent was pre incubated 10 minutes with a 10 fold excess of CD275(GL50,ICOSL)-mulg fusion protein (catalog #MBS666861).
Conjugation
Biotin
Production
Fusion protein from (low FBS containing) tissue culture supernatant of transfectants was purified using size exclusion chromatography.), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Preparation and Storage
Store at 2 - 5°C. Freeze/Thawing is not recommended.
Application Data
Application Data
Related Product Information for CD278 protein
Information: The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Each secondary signal from CD28 or ICOS results in a discrete cytokine secretion profile displayed by the activated T cell.1 Both activation processes are effectively down regulated by CD152 (CTLA-4) engagement.2 Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be a ligand for ICOS.3 ICOS-muIg recombinant protein binds to recombinant GL50-muIg in EIA.
References
Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717. 2 Riley, J.L., C.H. June, et al. 2001, J. Immunol. 166: 4943-4948. 3 Ling, V., M. Collins, et al. 2000, J. Immunol. 164: 1653-1657. 4 Ling, V., M. Collins, et al. 2001, J. Immunol. 166: 7300-7308. 5 A.J. McAdam, et al, (2000) J Immunol 165: 5035-5040.
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Product Notes
The CD278 (Catalog #AAA78190) is a Protein and is intended for research purposes only. The product is available for immediate purchase. The CD278(ICOS)-muIg-Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD278 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD278 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD278, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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