Application Data
(Recombinant IDH1 protein activity assay. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
IDH1 recombinant protein
Recombinant IDH1 protein
Synonyms
IDH1; N/A; Recombinant IDH1 protein; Isocitrate Dehydrogenase (NADP(+)) 1; Cytosolic; Oxalosuccinate Decarboxylase; NADP(+)-Specific ICDH; IDH1 recombinant protein
Host
E Coli
Form/Format
Recombinant IDH1 protein is supplied at a concentration of 0.8ug/ul in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol.
Protein Species
Human
Tag
His-Tag
Notes
This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant IDH1 protein activity assay. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
Application Data
(Recombinant IDH1 protein activity assay. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
Application Data
(Recombinant IDH1 protein activity assay. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
Application Data
(Recombinant IDH1 protein activity assay. 200 uM DL-isocitric acid trisodium and 200 uM NADP+ were incubated with IDH1 protein in reaction buffer including 50 mM Tris-HCl pH 7.4, 100 mM MgCl2 for 10 minutes at room temperature. The generation of product NADPH was monitored by measuring absorbance at 340 nm.)
SDS-PAGE
(Recombinant IDH1 protein, SDS-PAGE gel. Recombinant IDH1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 47.7 kDa Purity: > 90%)
SDS-PAGE
(Recombinant IDH1 protein, SDS-PAGE gel. Recombinant IDH1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 47.7 kDa Purity: > 90%)
Related Product Information for IDH1 recombinant protein
Short Description: Recombinant IDH1 protein was expressed in E Coli as the full-length protein (accession number AAH93020.1) with a C-terminal 6xHis-tag. The molecular weight of the protein is 47.7 kDa. It is suitable for peroxisome and NADPH regulation assays, as well as for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: IDH1 (Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic, also known as HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Isocitrate dehydrogenases belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Background: IDH1 (Isocitrate Dehydrogenase (NADP(+)) 1, Cytosolic, also known as HEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD) is a member of isocitrate dehydrogenases, which catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Isocitrate dehydrogenases belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Product Categories/Family for IDH1 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of the protein is 47.7kDa
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Product Notes
The IDH1 (Catalog #AAA59782) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IDH1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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