Standard Curve (Sample)
Rat IL-1 beta ELISA Kit | IL1B elisa kit
Rat IL-1 beta PicoKine ELISA Kit
Reactivity
Rat
Synonyms
IL-1 beta; N/A; Rat IL-1 beta PicoKine ELISA Kit; Interleukin-1 beta; interleukin 1, beta; Catabolin; Hematopoietin 1; IFN beta inducing factor; IL 1; IL 1 beta; IL 1B; IL1; IL1 BETA; IL1B; IL1B_HUMAN; IL1F2; Interleukin 1 beta; Interleukin 1 beta precursor; LAF; OAF; Osteoclast activating factor; Preinterleukin beta; Pro interleukin 1 beta; IL1B elisa kit
Reactivity
Rat
Specificity
Natural and recombinant Rat IL1B
Sequence Length
268
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL1B elisa kit
Principle of the Assay: The Picokine™ Rat IL1B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat IL1B with a 96-well strip plate that is pre-coated with antibody specific for IL1B. The detection antibody is a biotinylated antibody specific for IL1B. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Rat IL1B with immunogen: Expression system for standard: E.coli; Immunogen sequence: V117-S268. The kit is analytically validated with ready to use reagents. To measure Rat IL1B, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Rat IL1B in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat IL1B in the sample.
Background/Introduction: The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1(VCAM-1) on hepatic sinusoidal endothelium(HSE).
Background/Introduction: The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1(VCAM-1) on hepatic sinusoidal endothelium(HSE).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,659 Da
NCBI Official Full Name
Interleukin 1 beta
NCBI Official Synonym Full Names
interleukin 1 beta
NCBI Official Symbol
Il1b
NCBI Protein Information
interleukin-1 beta; IL-1 beta
UniProt Protein Name
Interleukin 1 beta
UniProt Gene Name
Il1b
UniProt Entry Name
Q5BKB0_RAT
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Product Notes
The Rat IL1B il1b (Catalog #AAA11564) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11564 ELISA Kit recognizes Rat IL1B. It is sometimes possible for the material contained within the vial of "IL-1 beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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