Standard Curve (Sample)
(Rat IL-4 PicoKine ELISA Kit standard curve)
Rat IL-4 ELISA Kit | IL4 elisa kit
Rat IL-4 PicoKine ELISA Kit
Gene Names
Il4; Il4e12
Reactivity
Rat
Synonyms
IL-4; N/A; Rat IL-4 PicoKine ELISA Kit; Interleukin-4; interleukin 4; B cell growth factor 1; B cell stimulatory factor 1; BCGF 1; BCGF1; Binetrakin; BSF 1; BSF1; IL 4; Interleukin 4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra; IL4 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat IL-4
Sequence Length
147
Samples
Cell culture supernates and serum
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 1pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
(Rat IL-4 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Rat IL-4 PicoKine ELISA Kit standard curve)
Related Product Information for IL4 elisa kit
Principle of the assay: rat IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-4 has been precoated onto 96-well plates. Standards(E.coli,C25-S147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-4 amount of sample captured in plate.
Background: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a proteinof 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functionalsimilarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123amino acids in mature form.
Background: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a proteinof 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functionalsimilarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123amino acids in mature form.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
16,247 Da
NCBI Official Full Name
Interleukin-4
NCBI Official Synonym Full Names
interleukin 4
NCBI Official Symbol
Il4
NCBI Official Synonym Symbols
Il4e12
NCBI Protein Information
interleukin-4; IL-4; BSF-1; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IGG differentiation factor
UniProt Protein Name
Interleukin-4
UniProt Gene Name
Il4
UniProt Synonym Gene Names
Il-4; IL-4; BSF-1
UniProt Entry Name
IL4_RAT
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Product Notes
The Rat IL4 il4 (Catalog #AAA45347) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45347 ELISA Kit recognizes Rat IL4. It is sometimes possible for the material contained within the vial of "IL-4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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