Standard Curve (Sample)
Mouse IL-6 ELISA Kit | IL6 elisa kit
Mouse IL-6 PicoKine ELISA Kit
Gene Names
Il6; Il-6
Reactivity
Mouse
Synonyms
IL-6; N/A; Mouse IL-6 PicoKine ELISA Kit; Interleukin-6; interleukin 6; B cell differentiation factor; B cell stimulatory factor 2; B-cell stimulatory factor 2; BSF 2; BSF-2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating fact; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFN-beta-2; IFNB2; IL 6; IL6; IL6 protein; IL6_HUMAN; Interferon beta 2; Interferon beta-2; Interleukin 6; Interleukin 6 (interferon beta 2); Interleukin BSF 2; Interleukin6; IL6 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse IL6
Sequence Length
211
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IL6 elisa kit
Background: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant IL-6, consisting of 188 amino acids with the molecular mass of 21.8kDa.
Principle of the Assay: The Picokine™ Mouse IL6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse IL6 with a 96-well strip plate that is pre-coated with antibody specific for IL6. The detection antibody is a biotinylated antibody specific for IL6. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit includes Mouse IL6 protein as standards. To measure Mouse IL6, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse IL6 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse IL6 in the sample.
Principle of the Assay: The Picokine™ Mouse IL6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse IL6 with a 96-well strip plate that is pre-coated with antibody specific for IL6. The detection antibody is a biotinylated antibody specific for IL6. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit includes Mouse IL6 protein as standards. To measure Mouse IL6, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse IL6 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse IL6 in the sample.
NCBI and Uniprot Product Information
NCBI GeneID
Molecular Weight
24,384 Da
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
Il-6
NCBI Protein Information
interleukin-6; interleukin HP-1; B-cell hybridoma growth factor
UniProt Protein Name
Interleukin 6
UniProt Gene Name
Il6
UniProt Entry Name
A2RTD1_MOUSE
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Product Notes
The Mouse IL6 il6 (Catalog #AAA11570) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11570 ELISA Kit recognizes Mouse IL6. It is sometimes possible for the material contained within the vial of "IL-6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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