WB (Western Blot)
(Western blot for Recombinant KAT1 / HAT1 protein activity 0.5 ug Histone H4 was incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant HAT1 / KAT1 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody . Western blot was used to detect the production of acetylated Histone H4.)
KAT1/HAT1 recombinant protein
Recombinant KAT1/HAT1 protein
Synonyms
KAT1/HAT1; N/A; Recombinant KAT1/HAT1 protein; Histone Acetyltransferase 1; EC 2.3.1.48; KAT1/HAT1 recombinant protein
Host
Baculovirus
Form/Format
Recombinant KAT1/HAT1 protein supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant KAT1/HAT1 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
WB (Western Blot)
(Western blot for Recombinant KAT1 / HAT1 protein activity 0.5 ug Histone H4 was incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant HAT1 / KAT1 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody . Western blot was used to detect the production of acetylated Histone H4.)
WB (Western Blot)
(Western blot for Recombinant KAT1 / HAT1 protein activity 0.5 ug Histone H4 was incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant HAT1 / KAT1 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody . Western blot was used to detect the production of acetylated Histone H4.)
SDS-PAGE
(Recombinant KAT1 / HAT1 protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 50.8 kDa Purity: >90%)
SDS-PAGE
(Recombinant KAT1 / HAT1 protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 50.8 kDa Purity: >90%)
Related Product Information for KAT1/HAT1 recombinant protein
Short Description: Full-length KAT1/HAT1 protein (accession number NP_003633.2) was expressed in a baculovirus expression system with an N-terminal DYKDDDDK tag. The molecular weight of KAT1/HAT1 is 50.8 kDa. Recombinant KAT1/HAT1 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: KAT1/HAT1 (Histone Acetyltransferase 1), is a type B histone acetyltransferase that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. It acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). It has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. HAT1 may be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. It may also play a role in DNA repair in response to free radical damage.
Background: KAT1/HAT1 (Histone Acetyltransferase 1), is a type B histone acetyltransferase that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. It acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). It has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. HAT1 may be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. It may also play a role in DNA repair in response to free radical damage.
Product Categories/Family for KAT1/HAT1 recombinant protein
NCBI and Uniprot Product Information
Molecular Weight
Molecular weight of KAT1/HAT1 is 50.8kDa
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Product Notes
The KAT1/HAT1 (Catalog #AAA60201) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "KAT1/HAT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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