SDS-PAGE
(The purity of KDM4B was determined to be >75% by densitometry.)
KDM4B recombinant protein
KDM4B recombinant protein
Gene Names
Kdm4b; Jmjd2b; mKIAA0876; 4732474L06Rik
Applications
Western Blot
Purity
>75%
Synonyms
KDM4B; N/A; KDM4B recombinant protein; Jmjd2b; mKIAA0876; 4732474L06Rik
Purity/Purification
>75%
Form/Format
50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1ug/ul (varies by lot)
Sequence Positions
1-725
Sequence Length
4610
Applicable Applications for KDM4B recombinant protein
WB (Western Blot)
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Shipping: Ships on Dry Ice
Shipping: Ships on Dry Ice
SDS-PAGE
(The purity of KDM4B was determined to be >75% by densitometry.)
SDS-PAGE
(The purity of KDM4B was determined to be >75% by densitometry.)
Related Product Information for KDM4B recombinant protein
Recombinant mouse KDM4B (1-725) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: KDM4B or lysine (K)-specific demethylase 4B, which is also known as JMJD2B, contains a JmjN domain, a JmjC domain, a JD2H domain, 2 TUDOR domains, and a bipartite nuclear localization signal that overlaps the C-terminal part of the second TUDOR domain. KDM4B plays an essential role in human carcinogenesis through positive regulation of cyclin-dependent kinase 6 (1). KDM4B functions as a co-factor of estrogen receptor in breast cancer proliferation and mammary gland development (2). KDM4B is regulated by both ERï¡ and HIF-1ï¡, drives breast cancer cell proliferation in normoxia and hypoxia, and epigenetically regulates the expression of cell cycle genes such as CCND1, CCNA1, and WEE1.
Scientific Background: KDM4B or lysine (K)-specific demethylase 4B, which is also known as JMJD2B, contains a JmjN domain, a JmjC domain, a JD2H domain, 2 TUDOR domains, and a bipartite nuclear localization signal that overlaps the C-terminal part of the second TUDOR domain. KDM4B plays an essential role in human carcinogenesis through positive regulation of cyclin-dependent kinase 6 (1). KDM4B functions as a co-factor of estrogen receptor in breast cancer proliferation and mammary gland development (2). KDM4B is regulated by both ERï¡ and HIF-1ï¡, drives breast cancer cell proliferation in normoxia and hypoxia, and epigenetically regulates the expression of cell cycle genes such as CCND1, CCNA1, and WEE1.
Product Categories/Family for KDM4B recombinant protein
References
1. Toyokawa G. et.al: The histone demethylase JMJD2B plays an essential role in human carcinogenesis through positive regulation of cyclin-dependent kinase 6. 2011 Dec.
2. Kawazu M. et.al: Histone demethylase JMJD2B functions as a co-factor of estrogen receptor in breast cancer proliferation and mammary gland development, 2011 Mar 18.
2. Kawazu M. et.al: Histone demethylase JMJD2B functions as a co-factor of estrogen receptor in breast cancer proliferation and mammary gland development, 2011 Mar 18.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
125 kDa
NCBI Official Full Name
Mus musculus lysine (K)-specific demethylase 4B (Kdm4b), mRNA
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4B
NCBI Official Symbol
Kdm4b
NCBI Official Synonym Symbols
Jmjd2b; mKIAA0876; 4732474L06Rik
NCBI Protein Information
lysine-specific demethylase 4B; jumonji domain containing 2B; jumonji domain-containing protein 2B; jmjC domain-containing histone demethylation protein 3B
UniProt Protein Name
Lysine-specific demethylase 4B
UniProt Gene Name
Kdm4b
UniProt Synonym Gene Names
Jhdm3b; Jmjd2b
UniProt Entry Name
KDM4B_MOUSE
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Product Notes
The KDM4B kdm4b (Catalog #AAA72911) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-725. AAA Biotech's KDM4B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the KDM4B kdm4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM4B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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