WB (Western Blot)
(Western blot analysis of K562 cell nuclear extract (40 ug) probed with KDM4B antibody (AAA50462) at a 1/500 dilution.)
Rabbit anti-Human KDM4B Polyclonal Antibody | anti-KDM4B antibody
KDM4B antibody
Gene Names
KDM4B; JMJD2B; TDRD14B
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunofluorescence
Purity
Antiserum to human KDM4B was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Synonyms
KDM4B, Antibody; KDM4B antibody; RABBIT ANTI HUMAN KDM4B; anti-KDM4B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antiserum to human KDM4B was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Applicable Applications for anti-KDM4B antibody
WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence)
Immunogen
A peptide within the C-terminal region of human KDM4B
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
WB (Western Blot)
(Western blot analysis of K562 cell nuclear extract (40 ug) probed with KDM4B antibody (AAA50462) at a 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of K562 cell nuclear extract (40 ug) probed with KDM4B antibody (AAA50462) at a 1/500 dilution.)
IP (Immunoprecipitation)
(Immunoprecipitation of KDM4B from K562 whole cell extract (lane 1). 4 ug of mouse IgG was used as a negative control (lane 2). Western blot analysis was performed using KDM4B antibody (AAA50462) at a 1/500 dilution.)
IP (Immunoprecipitation)
(Immunoprecipitation of KDM4B from K562 whole cell extract (lane 1). 4 ug of mouse IgG was used as a negative control (lane 2). Western blot analysis was performed using KDM4B antibody (AAA50462) at a 1/500 dilution.)
Related Product Information for anti-KDM4B antibody
Rabbit anti Human KDM4B antibody recognizes lysine-specific demethylase 4B, also known as JMJD2B.
KDM4B is a member of the alpha-ketoglutarate-dependent hydroxylase superfamily of enzymes. Alpha-ketoglutarate-dependent hydroxylases catalyse a wide range of reactions, including hydroxylation, demethylation and desaturation (Flashman and Schofield, 2007, Hausinger, 2004). KDM4B functions as a histone demethylase and is involved in the regulation of endothelial-to-mesenchymal transition (Glaser et al. 2020). KDM4B also forms a complex with MLL2 and acts as a co-factor of ERa-mediated transcription
KDM4B is a member of the alpha-ketoglutarate-dependent hydroxylase superfamily of enzymes. Alpha-ketoglutarate-dependent hydroxylases catalyse a wide range of reactions, including hydroxylation, demethylation and desaturation (Flashman and Schofield, 2007, Hausinger, 2004). KDM4B functions as a histone demethylase and is involved in the regulation of endothelial-to-mesenchymal transition (Glaser et al. 2020). KDM4B also forms a complex with MLL2 and acts as a co-factor of ERa-mediated transcription
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121,897 Da
NCBI Official Full Name
lysine-specific demethylase 4B
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4B
NCBI Official Symbol
KDM4B
NCBI Official Synonym Symbols
JMJD2B; TDRD14B
NCBI Protein Information
lysine-specific demethylase 4B; tudor domain containing 14B; jumonji domain containing 2B; jumonji domain-containing protein 2B; jmjC domain-containing histone demethylation protein 3B
UniProt Protein Name
Lysine-specific demethylase 4B
UniProt Gene Name
KDM4B
UniProt Synonym Gene Names
JHDM3B; JMJD2B; KIAA0876
UniProt Entry Name
KDM4B_HUMAN
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Product Notes
The KDM4B kdm4b (Catalog #AAA50462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KDM4B antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KDM4B can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence). Researchers should empirically determine the suitability of the KDM4B kdm4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDM4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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