Application Data
(Recombinant JMJD2D / KDM4D activity using AlphaLISA. JMJD2D / KDM4D was used in an AlphaLISA assay to determine enzyme titration. An IC50 dose response assessment of reference compounds 2,4-PDCA and N-OG is shown. This data was generated and kindly provided courtesy of ChemPartner.)
JMJD2D/KDM4D recombinant protein
Recombinant JMJD2D/KDM4D protein
Gene Names
KDM4D; JMJD2D
Purity
The recombinant protein is >60% pure by SDS-PAGE.
Synonyms
JMJD2D/KDM4D; N/A; Recombinant JMJD2D/KDM4D protein; Lysine Demethylase 4D; Jumonji Domain-Containing Protein 2D; JHDM3D; JMJD2D/KDM4D recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >60% pure by SDS-PAGE.
Form/Format
Full length recombinant JMJD2D/KDM4D protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant JMJD2D/KDM4D is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Histone Demethylase Assay Conditions: 50mM HEPES pH 7.5, 0.02% Triton X100, 100uM 2OG, 100uM Ascorbate, 50uM (NH4)2Fe(SO4)2·6H2O, 1mM TCEP, 100nm Recombinant JMJD2D/KDM4D protein, and 3.3uM H3K9me3 (aa 1-21) peptide at 2 hours at room temperature. MALDI-TOF was used for detection.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant JMJD2D / KDM4D activity using AlphaLISA. JMJD2D / KDM4D was used in an AlphaLISA assay to determine enzyme titration. An IC50 dose response assessment of reference compounds 2,4-PDCA and N-OG is shown. This data was generated and kindly provided courtesy of ChemPartner.)
Application Data
(Recombinant JMJD2D / KDM4D activity using AlphaLISA. JMJD2D / KDM4D was used in an AlphaLISA assay to determine enzyme titration. An IC50 dose response assessment of reference compounds 2,4-PDCA and N-OG is shown. This data was generated and kindly provided courtesy of ChemPartner.)
Application Data
(MALDI-TOF Assay for JMJD2D / KDM4D activity . 3.3 uM H3K9me3 peptide was incubated with 100 nM JMJD2D / KDM4D in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K9me3 peptide was used as a negative control.Catalytic Ability: >66 turnovers / enzyme molecule.)
Application Data
(MALDI-TOF Assay for JMJD2D / KDM4D activity . 3.3 uM H3K9me3 peptide was incubated with 100 nM JMJD2D / KDM4D in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K9me3 peptide was used as a negative control.Catalytic Ability: >66 turnovers / enzyme molecule.)
SDS-PAGE
(Recombinant JMJD2D / KDM4D protein gel. JMJD2D / KDM4D protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)
SDS-PAGE
(Recombinant JMJD2D / KDM4D protein gel. JMJD2D / KDM4D protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)
Related Product Information for JMJD2D/KDM4D recombinant protein
Short Description: Recombinant JMJD2D/KDM4D (accession number NP_060509.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 63.2 kDa. KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues. Recombinant JMJD2D/KDM4D can be used for enzyme kinetics and inhibitor screening studies.
Background: KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.
Background: KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.
Product Categories/Family for JMJD2D/KDM4D recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,603 Da
NCBI Official Full Name
lysine-specific demethylase 4D
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4D
NCBI Official Symbol
KDM4D
NCBI Official Synonym Symbols
JMJD2D
NCBI Protein Information
lysine-specific demethylase 4D; jumonji domain containing 2D; jumonji domain-containing protein 2D; jmjC domain-containing histone demethylation protein 3D
UniProt Protein Name
Lysine-specific demethylase 4D
UniProt Gene Name
KDM4D
UniProt Synonym Gene Names
JHDM3D; JMJD2D
UniProt Entry Name
KDM4D_HUMAN
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Product Notes
The JMJD2D/KDM4D kdm4d (Catalog #AAA59696) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JMJD2D/KDM4D, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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