DB (Dot Blot)
(AbFlex JMJD2D/KDM4D antibody tested by Dot blot. Recombinant KDM proteins were spotted onto nitrocellulose membrane (nanograms indicated on row labels) and probed with antibody at 1 ug/ml. Col. 1: KDM4A. Col. 2: KDM4B. Col. 3: KDM4C. Col. 4: KDM5A. Col. 5: KDM5B. Col. 6: KDM5C.)
Mouse anti-Human JMJD2D/KDM4D Antibody | anti-JMJD2D/KDM4D antibody
AbFlex JMJD2D/KDM4D antibody (rAb)
Gene Names
KDM4D; JMJD2D
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Dot Blot
Purity
Protein A Chromatography
Synonyms
JMJD2D/KDM4D; N/A; AbFlex JMJD2D/KDM4D antibody (rAb); Lysine Demethylase 4D; Jumonji Domain-Containing Protein 2D; JHDM3D; anti-JMJD2D/KDM4D antibody
Host
Mouse
Reactivity
Human
Isotype
IgG2a
Purity/Purification
Protein A Chromatography
Form/Format
140mM Hepes, pH7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-JMJD2D/KDM4D antibody
WB (Western Blot), IP (Immunoprecipitation), DB (Dot Blot)
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised against recombinant full-length human KDM4D (Lysine (K)-Specific Demethylase 4D).
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
DB (Dot Blot)
(AbFlex JMJD2D/KDM4D antibody tested by Dot blot. Recombinant KDM proteins were spotted onto nitrocellulose membrane (nanograms indicated on row labels) and probed with antibody at 1 ug/ml. Col. 1: KDM4A. Col. 2: KDM4B. Col. 3: KDM4C. Col. 4: KDM5A. Col. 5: KDM5B. Col. 6: KDM5C.)
DB (Dot Blot)
(AbFlex JMJD2D/KDM4D antibody tested by Dot blot. Recombinant KDM proteins were spotted onto nitrocellulose membrane (nanograms indicated on row labels) and probed with antibody at 1 ug/ml. Col. 1: KDM4A. Col. 2: KDM4B. Col. 3: KDM4C. Col. 4: KDM5A. Col. 5: KDM5B. Col. 6: KDM5C.)
IP (Immunoprecipitation)
(AbFlex JMJD2D/KDM4D antibody tested by Immunoprecipitation. 4 ug of AbFlex JMJD2D/KDM4 antibody (Lane1) or isotype control mouse IgG (Lane 2) was used to immunoprecipitate JMJD2D/KDM4D from 500 ug of HEK293 extract, which was then run on SDS-PAGE and probed with the same antibody at 2 u/ml. The appearance of antibody light chain at 25 kDa is observed as a cross-reactivity between the WB / IP antibody, and is common.)
IP (Immunoprecipitation)
(AbFlex JMJD2D/KDM4D antibody tested by Immunoprecipitation. 4 ug of AbFlex JMJD2D/KDM4 antibody (Lane1) or isotype control mouse IgG (Lane 2) was used to immunoprecipitate JMJD2D/KDM4D from 500 ug of HEK293 extract, which was then run on SDS-PAGE and probed with the same antibody at 2 u/ml. The appearance of antibody light chain at 25 kDa is observed as a cross-reactivity between the WB / IP antibody, and is common.)
SDS-PAGE
(AbFlex JMJD2D/KDM4D antibody tested by Western blot. 20 ug of HEK293 nuclear extract* was run on SDS-PAGE and probed with antibody at 0.5 ug/ml.)
SDS-PAGE
(AbFlex JMJD2D/KDM4D antibody tested by Western blot. 20 ug of HEK293 nuclear extract* was run on SDS-PAGE and probed with antibody at 0.5 ug/ml.)
Related Product Information for anti-JMJD2D/KDM4D antibody
Short Description: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. Our AbFlex JMJD2D/KDM4D antibody (rAb) was raised in a Mouse host. It has been validated for use in Dot blot, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2D/KDM4D antibody was expressed in was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.
Background: AbFlex antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-tag, a Biotinylation Tag for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex JMJD2D/KDM4D antibody was expressed in was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. KDM4D (lysine (K)-specific demethylase 4D), also known as JMJD2D (Jumonji Domain Containing 2D) is a protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 residues of histone H3, while it has no activity on monomethylated H3K9 residues.
Product Categories/Family for anti-JMJD2D/KDM4D antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,603 Da
NCBI Official Full Name
lysine-specific demethylase 4D
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4D
NCBI Official Symbol
KDM4D
NCBI Official Synonym Symbols
JMJD2D
NCBI Protein Information
lysine-specific demethylase 4D; jmjC domain-containing histone demethylation protein 3D; jumonji domain containing 2D; jumonji domain-containing protein 2D
UniProt Protein Name
Lysine-specific demethylase 4D
UniProt Gene Name
KDM4D
UniProt Synonym Gene Names
JHDM3D; JMJD2D
UniProt Entry Name
KDM4D_HUMAN
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Product Notes
The JMJD2D/KDM4D kdm4d (Catalog #AAA60304) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AbFlex JMJD2D/KDM4D antibody (rAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD2D/KDM4D can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), DB (Dot Blot). Researchers should empirically determine the suitability of the JMJD2D/KDM4D kdm4d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD2D/KDM4D, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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