Application Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JARID1A/KDM5A recombinant protein
Recombinant JARID1A/KDM5A protein
Gene Names
KDM5A; RBP2; RBBP2; RBBP-2
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Synonyms
JARID1A/KDM5A; N/A; Recombinant JARID1A/KDM5A protein; RBP2; RBBP2; RBBP-2; JARID1A/KDM5A recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >85% pure by SDS-PAGE.
Form/Format
Full length recombinant JARID1A/KDM5A protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant JARID1A / KDM5A protein gel. JARID1A / KDM5A protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 196 kDa Purity: >60%)
SDS-PAGE
(Recombinant JARID1A / KDM5A protein gel. JARID1A / KDM5A protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 196 kDa Purity: >60%)
Related Product Information for JARID1A/KDM5A recombinant protein
Short Description: Recombinant JARID1A/KDM5A (accession number NP_001036068.1) was expressed in Sf9 and contains an N-terminal DYKDDDDK tag with a molecular weight of 196.3 kDa. Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis. Recombinant JARID1A/KDM5A can be used for enzyme kinetics and inhibitor screening studies.
Background: Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis.
Background: Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis.
Product Categories/Family for JARID1A/KDM5A recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
186,289 Da
NCBI Official Full Name
lysine-specific demethylase 5A
NCBI Official Synonym Full Names
lysine demethylase 5A
NCBI Official Symbol
KDM5A
NCBI Official Synonym Symbols
RBP2; RBBP2; RBBP-2
NCBI Protein Information
lysine-specific demethylase 5A
UniProt Protein Name
Lysine-specific demethylase 5A
UniProt Gene Name
KDM5A
UniProt Synonym Gene Names
JARID1A; RBBP2; RBP2; RBBP-2
UniProt Entry Name
KDM5A_HUMAN
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Product Notes
The JARID1A/KDM5A kdm5a (Catalog #AAA59679) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JARID1A/KDM5A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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