NKp80/KLRF1 Recombinant Protein | KLRF1 recombinant protein

Recombinant Human NKp80/KLRF1 Protein (hFc Tag), HPLC-verified

Cat. Num. AAA259285

• Gene Names: KLRF1; NKp80; CLEC5C
• Reactivity: Human
• Purity: >= 90% as determined by SDS-PAGE. >= 90% as determined by SEC-HPLC.
• Synonyms: NKp80/KLRF1; N/A; Recombinant Human NKp80/KLRF1 Protein (hFc Tag), HPLC-verified; Human NKp80/KLRF1 Protein (Fc Tag); killer cell lectin like receptor F1; CLEC5C Protein, Human; ML Protein, Human; NKp80 Protein, Human; KLRF1 recombinant protein

• Host: HEK293 Cells
• Reactivity: Human
• Purity/Purification: >= 90% as determined by SDS-PAGE. >= 90% as determined by SEC-HPLC.
• Form/Format: Lyophilized from sterile 20 mM MES, 150 mM NaCl, 8% Glycerol, 10% trehalose, pH 6.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence: Val66-Tyr231

• Tag: N-human IgG1-Fc
• Predicted N Terminal: Glu
• Endotoxin: < 1.0 EU perug of the protein as determined by the LAL method
• Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells (Routinely tested).
• Protein Construction: A DNA sequence encoding the Human KLRF1 (NP_057607.1)(Val66-Tyr231) was expressed with the Fc region of human IgG1 at the N-terminus.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Application Data

Application Data

Related Product Information for KLRF1 recombinant protein

NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor that is expressed on nearly all-natural killer cells and stimulates their cytotoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y3F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the Hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp8, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-7 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the Hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical Hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.

Product Categories/Family for KLRF1 recombinant protein

ITIM/ITAM Immunoreceptors and Related Molecules

References

Kuttruf. S. e. al.. 2009. Blood.11. (2). 358-69. Denneh. KM. e. al.. 2011. . Immunol. 18. (2). 657-61. Roda-Navarr. P. e. al.. 2000. Eu. . Immunol. 3. (2). 568-76.

NCBI and Uniprot Product Information

• NCBI GI #: 7705574
• NCBI GeneID: 51348
• NCBI Accession #: NP_057607.1
• NCBI GenBank Nucleotide #: NM_016523.1
• UniProt Accession #: Q9NZS2; Q4KMT5; Q96PR2; Q96PR3; Q9NZS1
• Molecular Weight: 26,666 Da
• NCBI Official Full Name: killer cell lectin-like receptor subfamily F member 1
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily F, member 1
• NCBI Official Symbol: KLRF1
• NCBI Official Synonym Symbols: NKp80; CLEC5C
• NCBI Protein Information: killer cell lectin-like receptor subfamily F member 1; lectin-like receptor F1; activating coreceptor NKp80; killer cell lectin-like receptor F1; C-type lectin domain family 5 member C
• UniProt Protein Name: Killer cell lectin-like receptor subfamily F member 1
• UniProt Gene Name: KLRF1
• UniProt Synonym Gene Names: CLEC5C; ML; Lectin-like receptor F1
• UniProt Entry Name: KLRF1_HUMAN