Application Data
(Recombinant Human NKp80/KLRF1 Protein, His Tag, HPLC-verified.)
NKp80/KLRF1 Recombinant Protein | KLRF1 recombinant protein
Recombinant Human NKp80/KLRF1 Protein (His Tag), HPLC-verified
Gene Names
KLRF1; NKp80; CLEC5C
Reactivity
Human
Purity
>=95% as determined by SDS-PAGE. >= 90% as determined by SEC-HPLC. >= 90% as determined by SEC-MALS (Routinely tested)
Synonyms
NKp80/KLRF1; N/A; Recombinant Human NKp80/KLRF1 Protein (His Tag), HPLC-verified; Human NKp80/KLRF1 Protein (His Tag); killer cell lectin like receptor F1; CLEC5C Protein, Human; ML Protein, Human; NKp80 Protein, Human; KLRF1 recombinant protein
Host
HEK293 Cells
Reactivity
Human
Purity/Purification
>=95% as determined by SDS-PAGE. >= 90% as determined by SEC-HPLC. >= 90% as determined by SEC-MALS (Routinely tested)
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Sequence
Leu60-Tyr231
Tag
N-His
Predicted N Terminal
His
Endotoxin
< 1.0 EU perug of the protein as determined by the LAL method
Protein Construction
A DNA sequence encoding the Human KLRF1 (NP_057607.1)(Leu60-Tyr231) was expressed, with a polyhistidine tag at the N-terminus.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Application Data
(Recombinant Human NKp80/KLRF1 Protein, His Tag, HPLC-verified.)
Application Data
(Recombinant Human NKp80/KLRF1 Protein, His Tag, HPLC-verified.)
Application Data
Application Data
Application Data
Application Data
Related Product Information for KLRF1 recombinant protein
NKp80, also known as KLRF1, is an activating homodimeric C-type lectin-like receptor that is expressed on nearly all-natural killer cells and stimulates their cytotoxicity and cytokine release. NKp80 stimulates cytotoxicity upon engagement of its genetically linked ligand: myeloid-specific CTLR activation-induced C-type lectin (AICL). NKp80, but not NKp80 mutated at tyrosine 7 (NKp80/Y7F), is tyrosine phosphorylated. Accordingly, NKp80/Y7F, but not NKp80/Y3F or NKp80/Y37F, failed to induce cytotoxicity. NKp80 phosphopeptides comprising the Hemi-ITAM-like sequence surrounding tyrosine 7 bound Lck- and Syk-family kinases; accordingly, cross-linking of NKp8, but not NKp80/Y7F, induced Syk phosphorylation. Moreover, inhibition of Syk kinase, but not ZAP-7 kinase, impaired cytotoxic responses through NKp80. Atypical residues in the Hemi-ITAM-like motif of NKp80 cause an altered stoichiometry of phosphorylation but did not substantially affect NK cytotoxicity. Altogether, these results show that NKp80 uses an atypical Hemi-ITAM and Syk kinase to trigger cellular cytotoxicity.
Product Categories/Family for KLRF1 recombinant protein
References
Kuttruf. S. e. al.. 2009. Blood.11. (2). 358-69. Denneh. KM. e. al.. 2011. . Immunol. 18. (2). 657-61. Roda-Navarr. P. e. al.. 2000. Eu. . Immunol. 3. (2). 568-76.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,666 Da
NCBI Official Full Name
killer cell lectin-like receptor subfamily F member 1
NCBI Official Synonym Full Names
killer cell lectin-like receptor subfamily F, member 1
NCBI Official Symbol
KLRF1
NCBI Official Synonym Symbols
NKp80; CLEC5C
NCBI Protein Information
killer cell lectin-like receptor subfamily F member 1; lectin-like receptor F1; activating coreceptor NKp80; killer cell lectin-like receptor F1; C-type lectin domain family 5 member C
UniProt Protein Name
Killer cell lectin-like receptor subfamily F member 1
UniProt Gene Name
KLRF1
UniProt Synonym Gene Names
CLEC5C; ML; Lectin-like receptor F1
UniProt Entry Name
KLRF1_HUMAN
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Product Notes
The KLRF1 klrf1 (Catalog #AAA259286) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human NKp80/KLRF1 Protein (His Tag), HPLC-verified reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: Leu60-Tyr2 31. It is sometimes possible for the material contained within the vial of "NKp80/KLRF1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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