NKp80 Antibody | anti-NKp80 antibody

Anti-Hu NKp80 Purified

Cat. Num. AAA129205

• Gene Names: KLRF1; NKp80; CLEC5C
• Reactivity: Human, Monkey
• Applications: Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: NKp80; N/A; Anti-Hu NKp80 Purified; killer cell lectin like receptor F1; KLRF1; CLEC5C; anti-NKp80 antibody

• Reactivity: Human, Monkey
• Isotype: Mouse IgG1 kappa
• Clone Number: 5D12
• Specificity: The mouse monoclonal antibody 5D12 recognizes an extracellular epitope of human NKp80 (CLEC5C), a C-type lectin family member, expressed on NK cells and subsets of T cells.
• Form/Format: Purified; Purified by protein-A affinity chromatography.; Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NKp80 antibody: IP (Immunoprecipitation), FCM/FACS (Flow Cytometry)
• Immunogen: recombinant human NKp80 extracellular domain
• Negative species: Mouse, Rat
• Preparation and Storage: Store at 2-8 degree C. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human NKp80 positive cells (red-filled) from human NKp80 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human NKp80 (5D12) purified antibody (concentration in sample 1,7 ?g/ml, GAM APC).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human NKp80 (5D12) purified antibody (concentration in sample 1,7 ?g/ml, GAM APC).)

Related Product Information for anti-NKp80 antibody

NKp80, also known as CLEC5C or KLRF1, is a type II transmembrane glycoprotein of the C lectin family, which is expressed in 80 kDa homodimers on NK cells, and subsets of CD8+ alpha/beta T cells, and gamma/delta T cells. It belongs to the activating coreceptors, which induce cytotoxicity, and production of pro-inflammatory cytokines. Its ligand AICL is expressed on myeloid cells.

References

*Dennehy KM, Klimosch SN, Steinle A: Cutting edge: NKp80 uses an atypical hemi-ITAM to trigger NK cytotoxicity. J Immunol. 2011 Jan 15;186(2):657-61., URL: https://pubmed.ncbi.nlm.nih.gov/21149606/,*Campbell TM, McSharry BP, Steain M, Russell TA, Tscharke DC, Kennedy JJ, Slobedman B, Abendroth A: Functional paralysis of human natural killer cells by alphaherpesviruses. PLoS Pathog. 2019 Jun 13;15(6):e1007784, URL: https://pubmed.ncbi.nlm.nih.gov/31194857/,*Rückrich T, Steinle A: Attenuated natural killer (NK) cell activation through C-type lectin-like receptor NKp80 is due to an anomalous hemi-immunoreceptor tyrosine-based activation motif (HemITAM) with impaired Syk kinase recruitment capacity. J Biol Chem. 2013 Jun 14;288(24):17725-33., URL: https://pubmed.ncbi.nlm.nih.gov/23609447/,

NCBI and Uniprot Product Information

• NCBI GI #: 7705574
• NCBI GeneID: 51348
• NCBI Accession #: NP_057607.1
• NCBI GenBank Nucleotide #: NM_016523.1
• UniProt Accession #: Q9NZS2; Q4KMT5; Q96PR2; Q96PR3; Q9NZS1
• Molecular Weight: 26,666 Da
• NCBI Official Full Name: killer cell lectin-like receptor subfamily F member 1
• NCBI Official Synonym Full Names: killer cell lectin-like receptor subfamily F, member 1
• NCBI Official Symbol: KLRF1
• NCBI Official Synonym Symbols: NKp80; CLEC5C
• NCBI Protein Information: killer cell lectin-like receptor subfamily F member 1; lectin-like receptor F1; activating coreceptor NKp80; killer cell lectin-like receptor F1; C-type lectin domain family 5 member C
• UniProt Protein Name: Killer cell lectin-like receptor subfamily F member 1
• UniProt Gene Name: KLRF1
• UniProt Synonym Gene Names: CLEC5C; ML; Lectin-like receptor F1
• UniProt Entry Name: KLRF1_HUMAN