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product-image-AAA111631_IF11.jpg IF (Immunofluorescence) (Peptide-+ Immunofluorescence analysis of HeLa cells, using Keratin 8 (Ab-73) antibody.)

anti-Human, Mouse Keratin 8 Antibody | anti-KRT8 antibody

Keratin 8 (Ab-73) Antibody

Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Human, Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Keratin 8; N/A; Keratin 8 (Ab-73) Antibody; CK 8; CK8; CYK8; Cytokeratin endo A; K2C8; K8; KRT2-8; Keratin; type II cytoskeletal 8; cytokeratin 8; anti-KRT8 antibody
Ordering
Reactivity
Human, Mouse
Specificity
Keratin 8 (Ab-73) antibody detects endogenous levels of total Keratin 8 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
483
Applicable Applications for anti-KRT8 antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human Keratin 8 around the phosphorylation site of serine 73 (L-L-SP-P-L).
Preparation and Storage
Store at-20 degree C for 1 year

IF (Immunofluorescence)

(Peptide-+ Immunofluorescence analysis of HeLa cells, using Keratin 8 (Ab-73) antibody.)

product-image-AAA111631_IF11.jpg IF (Immunofluorescence) (Peptide-+ Immunofluorescence analysis of HeLa cells, using Keratin 8 (Ab-73) antibody.)

WB (Western Blot)

(Western blot analysis of extracts from Hela cells treated with Anisomycin (25ug/ml, 30min), using Keratin 8 (Ab-73) antibody.)

product-image-AAA111631_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from Hela cells treated with Anisomycin (25ug/ml, 30min), using Keratin 8 (Ab-73) antibody.)

IHC (Immunohistochemistry)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (Ab-73) antibody.)

product-image-AAA111631_IHC15.jpg IHC (Immunohistochemistry) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Keratin 8 (Ab-73) antibody.)
References
Nam-On Ku J. Biol. Chem., Mar 2002; 277: 10775-10782. Karen M. Ridge J. Biol. Chem., Aug 2005; 280: 30400-30405. Nam-On Ku J. Biol. Chem., Mar 1997; 272: 7556. Gary K. Scott Mol. Pharmacol., Sep 2005; 68: 606-615.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,608 Da
NCBI Official Full Name
keratin, type II cytoskeletal 8 isoform 2
NCBI Official Synonym Full Names
keratin 8
NCBI Official Symbol
KRT8
NCBI Official Synonym Symbols
K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
NCBI Protein Information
keratin, type II cytoskeletal 8
UniProt Protein Name
Keratin, type II cytoskeletal 8
UniProt Gene Name
KRT8
UniProt Synonym Gene Names
CYK8; CK-8; K8

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Product Notes

The KRT8 krt8 (Catalog #AAA111631) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Keratin 8 (Ab-73) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 8 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KRT8 krt8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 8, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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