Application Data
(HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
LCK recombinant protein
Recombinant LCK protein
Gene Names
LCK; LSK; YT16; p56lck; pp58lck
Synonyms
LCK; N/A; Recombinant LCK protein; LCK Proto-Oncogene; Src Family Tyrosine Kinase; LCK recombinant protein
Host
Baculovirus
Form/Format
Recombinant LCK protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Concentration
0.15 ug/ul (varies by lot)
Protein Details
Recombinant LCK protein was expressed in baculovirus expression system as the full length protein (accession number NP_001036236.1) with a N-terminal DYKDDDDK tag.
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant LCK protein 10% SDS-PAGE with Coomassie staining MW: 59.3 kDa Purity: >90%)
SDS-PAGE
(Recombinant LCK protein 10% SDS-PAGE with Coomassie staining MW: 59.3 kDa Purity: >90%)
Related Product Information for LCK recombinant protein
Background: LCK (LCK Proto-Oncogene, Src Family Tyrosine Kinase) is a non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. It plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP.
Product Categories/Family for LCK recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59.3 kDa
NCBI Official Full Name
tyrosine-protein kinase Lck
NCBI Official Synonym Full Names
lymphocyte-specific protein tyrosine kinase
NCBI Official Symbol
LCK
NCBI Official Synonym Symbols
LSK; YT16; p56lck; pp58lck
NCBI Protein Information
tyrosine-protein kinase Lck; leukocyte C-terminal Src kinase; p56(LSTRA) protein-tyrosine kinase; t cell-specific protein-tyrosine kinase; proto-oncogene tyrosine-protein kinase LCK; lymphocyte cell-specific protein-tyrosine kinase; T-lymphocyte specific
UniProt Protein Name
Tyrosine-protein kinase Lck
UniProt Gene Name
LCK
UniProt Synonym Gene Names
LSK
UniProt Entry Name
LCK_HUMAN
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Product Notes
The LCK lck (Catalog #AAA60254) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LCK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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