Sequence
Lens Epithelium Derived Growth Factor (LEDGF) Recombinant Protein | LEDGF recombinant protein
Recombinant Lens Epithelium Derived Growth Factor (LEDGF)
Gene Names
PSIP1; p52; p75; PAIP; DFS70; LEDGF; PSIP2
Applications
Western Blot, SDS-Page
Purity
> 90%
Synonyms
Lens Epithelium Derived Growth Factor (LEDGF); N/A; Recombinant Lens Epithelium Derived Growth Factor (LEDGF); LEDGF recombinant protein
Host
E.coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
150ug/mL (varies by lot)
Sequence Positions
Met1~Asn64
Applicable Applications for LEDGF recombinant protein
WB (Western Blot), SDS-PAGE, Immunogen, Positive Control
Source
Prokaryotic expression
Tags
N-terminal His and GST Tag
Subcellular Location
Nucleus
Traits
Freeze-dried powder
Predicted isoelectric point
7.7
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Identification
(Gene Sequencing)
Identification
(Gene Sequencing)
SDS-PAGE
SDS-PAGE
Related Product Information for LEDGF recombinant protein
Predicted Molecular Mass: 37.3kDa
Accurate Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.
Product Categories/Family for LEDGF recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted MW: 37.3 kDa
Accurate MW: 42 kDa as determined by SDS-PAGE reducing conditions.
Accurate MW: 42 kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
lens epithelium-derived growth factor
NCBI Official Synonym Full Names
PC4 and SFRS1 interacting protein 1
NCBI Official Symbol
PSIP1
NCBI Official Synonym Symbols
p52; p75; PAIP; DFS70; LEDGF; PSIP2
NCBI Protein Information
PC4 and SFRS1-interacting protein
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Product Notes
The LEDGF (Catalog #AAA149300) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Met1~Asn64. AAA Biotech's Lens Epithelium Derived Growth Factor (LEDGF) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), SDS-PAGE, Immunogen, Positive Control. Researchers should empirically determine the suitability of the LEDGF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lens Epithelium Derived Growth Factor (LEDGF), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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