IP (Immunoprecipitation)
(Detection of human LEDGF/p75 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF/p75 antibody AAA211178 (lot AAA211178-3) used for IP at 3 ug per reaction. LEDGF/p75 was also immunoprecipitated by rabbit anti-LEDGF/p75 antibody For blotting immunoprecipitated LEDGF-p75, AAA211178 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human, Mouse LEDGF/p75 Polyclonal Antibody | anti-LEDGF/p75 antibody
Rabbit anti-LEDGF/p75 Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
LEDGF/p75, Antibody; Rabbit anti-LEDGF/p75 Antibody, Affinity Purified; PAIP; p52; Transcriptional coactivator p75/p52; transcriptional coactivator p52/p75; PC4 and SFRS1-interacting protein; p75; LEDGF; CLL-associated antigen KW-7; DFS70; DFS 70; dense fine speckles 70 kDa protein; PSIP2; lens epithelium-derived growth factor; PC4 and SFRS1 interacting protein 1; anti-LEDGF/p75 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-LEDGF/p75 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Cat
Immunogen
Between 225 and 275
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human LEDGF/p75 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF/p75 antibody AAA211178 (lot AAA211178-3) used for IP at 3 ug per reaction. LEDGF/p75 was also immunoprecipitated by rabbit anti-LEDGF/p75 antibody For blotting immunoprecipitated LEDGF-p75, AAA211178 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human LEDGF/p75 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF/p75 antibody AAA211178 (lot AAA211178-3) used for IP at 3 ug per reaction. LEDGF/p75 was also immunoprecipitated by rabbit anti-LEDGF/p75 antibody For blotting immunoprecipitated LEDGF-p75, AAA211178 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry)
(Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (AAA211178 lot 3) used at 1:5000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (AAA211178 lot 3) used at 1:5000 (0.2 ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Related Product Information for anti-LEDGF/p75 antibody
LEDGF/p75 has been identified as a transcriptional coactivator involved in lens epithelial cell gene regulation and in neuroepithelial stem cell differentiation and neurogenesis. LEDGF has also been shown to function as a cofactor for lentivirus and HIV integration.
Product Categories/Family for anti-LEDGF/p75 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The LEDGF/p75 psip1 (Catalog #AAA211178) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-LEDGF/p75 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LEDGF/p75 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the LEDGF/p75 psip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEDGF/p75, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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