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Application Data
Mouse LEDGF Monoclonal Antibody | anti-LEDGF antibody
LEDGF Monoclonal Antibody
Gene Names
PSIP1; p52; p75; PAIP; DFS70; LEDGF; PSIP2
Reactivity
Human, Rat, Monkey
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
LEDGF, Antibody; LEDGF Monoclonal Antibody; PC4 and SFRS1-interacting protein; PSIP1; DFS70; LEDGF; PSIP2; CLL-associated antigen KW-7; Dense fine speckles 70 kDa protein; DFS 70; Lens epithelium-derived growth factor; Transcriptional coactivator p75/p52; anti-LEDGF antibody
Host
Mouse
Reactivity
Human, Rat, Monkey
Clonality
Monoclonal
Specificity
LEDGF Monoclonal Antibody detects endogenous levels of LEDGF protein.
Purity/Purification
Affinity purification
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-LEDGF antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Purified recombinant fragment of human LEDGF expressed in E coli.
Modification
Unmodified/Total
Disease
A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
Domain
Residues 340-417 are necessary and sufficient for the interaction with HIV-1 IN (IBD domain).
Function
Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.
PTM
Phosphorylated upon DNA damage, probably by ATM or ATR.
Belongs to the HDGF family.
Contains 1 PWWP domain.
Belongs to the HDGF family.
Contains 1 PWWP domain.
Subcellular Location
Remains chromatin-associated throughout the cell cycle.
Subunit
Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts with lentiviral integrase and acts as a chromatin tethering factor to the chromosomal DNA. Isoform 1 interacts in particular with the human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome. Isoform 1 interacts also with HIV-2 IN. Isoform 2 does not interact with HIV-1 IN.
Tissue Specificity
Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions.
Preparation and Storage
Store at -20 degree C/1 year
Application Data
Application Data
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using LEDGF Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using LEDGF Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using LEDGF Monoclonal Antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded breast cancer tissues (left) and ovarian cancer tissues (right) with DAB staining using LEDGF Monoclonal Antibody.)
WB (Western Blot)
(Western Blot analysis using LEDGF Monoclonal Antibody against HepG2 (1). Jurkat (2). K562 (3). Cos7 (4). PC-12 (5). HeLa (6). and NIH/3T3 (7) cell lysate.)
WB (Western Blot)
(Western Blot analysis using LEDGF Monoclonal Antibody against HepG2 (1). Jurkat (2). K562 (3). Cos7 (4). PC-12 (5). HeLa (6). and NIH/3T3 (7) cell lysate.)
Product Categories/Family for anti-LEDGF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,268 Da
NCBI Official Full Name
PC4 and SFRS1-interacting protein isoform 2
NCBI Official Synonym Full Names
PC4 and SFRS1 interacting protein 1
NCBI Official Symbol
PSIP1
NCBI Official Synonym Symbols
p52; p75; PAIP; DFS70; LEDGF; PSIP2
NCBI Protein Information
PC4 and SFRS1-interacting protein
UniProt Protein Name
PC4 and SFRS1-interacting protein
UniProt Gene Name
PSIP1
UniProt Synonym Gene Names
DFS70; LEDGF; PSIP2; DFS 70
UniProt Entry Name
PSIP1_HUMAN
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Product Notes
The LEDGF psip1 (Catalog #AAA320139) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LEDGF Monoclonal Antibody reacts with Human, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's LEDGF can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the LEDGF psip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEDGF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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