Standard Curve (Sample)
Human LIM homeobox transcription factor 1-beta ELISA Kit | LMX1B elisa kit
Human LIM homeobox transcription factor 1-beta ELISA Kit
Gene Names
LMX1B; NPS1; LMX1.2
Reactivity
Human
Synonyms
LIM homeobox transcription factor 1-beta; N/A; Human LIM homeobox transcription factor 1-beta ELISA Kit; LMX1B (LIM homeobox transcription factor 1-beta)/LMX-1.2/LIM,homeobox protein 1.2/LIM,homeobox protein LMX1B; LMX1B elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of LMX1B. No significant cross-reactivity or interference between LMX1B and analogues was observed.
Sequence Length
402
Assay Type
Competitive ELISA, Coated with Antigen
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LMX1B were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LMX1B were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LMX1B elisa kit
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with LMX1B. During the reaction, LMX1B in the sample or standard competes with a fixed amount of LMX1B on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to LMX1B. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LMX1B in the samples is then determined by comparing the OD. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45,080 Da
NCBI Official Full Name
LIM homeobox transcription factor 1-beta isoform 2
NCBI Official Synonym Full Names
LIM homeobox transcription factor 1 beta
NCBI Official Symbol
LMX1B
NCBI Official Synonym Symbols
NPS1; LMX1.2
NCBI Protein Information
LIM homeobox transcription factor 1-beta
UniProt Protein Name
LIM homeobox transcription factor 1-beta
UniProt Gene Name
LMX1B
UniProt Synonym Gene Names
LMX-1.2
UniProt Entry Name
LMX1B_HUMAN
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Product Notes
The Human LMX1B lmx1b (Catalog #AAA97724) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA97724 ELISA Kit recognizes Human LMX1B. It is sometimes possible for the material contained within the vial of "LIM homeobox transcription factor 1-beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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