Prolactin receptor (PRLR) Active Protein | PRLR active protein

Recombinant Human Prolactin receptor (PRLR), partial (Active)

Cat. Num. AAA278911

• Gene Names: PRLR; hPRLrI
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Prolactin receptor (PRLR); N/A; Recombinant Human Prolactin receptor (PRLR), partial (Active); (PRL-R); PRLR active protein

• Host: Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 25-234aa, Partial
• Sequence: QLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMND

• Species: Homo sapiens (Human)
• Endotoxin: Less than 1.0EU/ug as determined by LAL method.
• Relevance: This is a receptor for the anterior pituitary hormone prolactin (PRL). Acts as a prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT. Isoform 4 is unable to transduce prolactin signaling. Isoform 6 is unable to transduce prolactin signaling.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human PRLR at 2ug/mL can bind Anti-PRLR recombinant antibody , the EC50 is 126.8-171.9ng/mL.
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.; Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Bioactivity

SDS-PAGE

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

NCBI and Uniprot Product Information

• NCBI GI #: 4506107
• NCBI GeneID: 5618
• NCBI Accession #: NP_000940.1
• NCBI GenBank Nucleotide #: NM_000949.5
• UniProt Accession #: P16471; Q16354; Q8TD75; Q8TD78; Q96P35; Q96P36; Q9BX87; Q9UHJ5; B2R882
• Molecular Weight: 24,000
• NCBI Official Full Name: prolactin receptor isoform 1
• NCBI Official Synonym Full Names: prolactin receptor
• NCBI Official Symbol: PRLR
• NCBI Official Synonym Symbols: hPRLrI
• NCBI Protein Information: prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein
• UniProt Protein Name: Prolactin receptor
• UniProt Gene Name: PRLR
• UniProt Synonym Gene Names: PRL-R
• UniProt Entry Name: PRLR_HUMAN